PIP2 Antibody (PIP2 2C11): sc-53412

PIP2 Antibody (PIP2 2C11) is a mouse monoclonal IgM antibody that detects PIP2 protein of mouse, rat, and human origin by immunoprecipitation (IP) and immunofluorescence (IF). Anti-PIP2 antibody (2C11) is available as a non-conjugated format. Phosphatidylinositol 4,5-bisphosphate (PIP2) is a crucial low-abundance membrane phospholipid that plays a significant role in various cellular processes, including membrane trafficking, plasma membrane-cytoskeleton linkages, and synaptic vesicle recycling. PIP2′s importance extends to second messenger signaling, cell adhesion, motility, and regulation of proteins involved in phospholipid metabolism. PIP2 stimulates Ca2+-activated ATPase, suggesting that fluctuations in cellular PIP2 concentrations may be vital for regulating calcium transport within the endoplasmic reticulum. PIP2 interacts with the taste transduction ion channel TRPM5, highlighting a role in sensory signaling pathways. Hormone-regulated PIP2 levels serve as an effector for multiple downstream proteins, indicating a central role in cellular signaling networks. PIP2 may act as a second messenger in controlling cytoskeleton-membrane adhesion, underscoring multifaceted functions in maintaining cellular integrity and communication.

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PIP2 Antibody (PIP2 2C11) References:

1. PIP2 and PIP3: complex roles at the cell surface.  |  Czech, MP. 2000. Cell. 100: 603-6. PMID: 10761925
2. Generation of phosphatidylinositol-specific antibodies and their characterization.  |  Thomas, CL., et al. 1999. Biochem Soc Trans. 27: 648-52. PMID: 10917659
3. Nuclear PtdIns(4,5)P2 assembles in a mitotically regulated particle involved in pre-mRNA splicing.  |  Osborne, SL., et al. 2001. J Cell Sci. 114: 2501-11. PMID: 11559758
4. Intracellular Ca2+ and the phospholipid PIP2 regulate the taste transduction ion channel TRPM5.  |  Liu, D. and Liman, ER. 2003. Proc Natl Acad Sci U S A. 100: 15160-5. PMID: 14657398
5. PIP2 regulation of KCNQ channels: biophysical and molecular mechanisms for lipid modulation of voltage-dependent gating.  |  Zaydman, MA. and Cui, J. 2014. Front Physiol. 5: 195. PMID: 24904429
6. Binding of neomycin to phosphatidylinositol 4,5-bisphosphate (PIP2).  |  Gabev, E., et al. 1989. Biochim Biophys Acta. 979: 105-12. PMID: 2537103
7. The mechanism of Gαq regulation of PLCβ3-catalyzed PIP2 hydrolysis.  |  Falzone, ME. and MacKinnon, R. 2023. Proc Natl Acad Sci U S A. 120: e2315011120. PMID: 37991948
8. LRRC8A as a central mediator promotes colon cancer metastasis by regulating PIP5K1B/PIP2 pathway.  |  Zhang, H., et al. 2024. Biochim Biophys Acta Mol Basis Dis. 1870: 167066. PMID: 38350542
9. PIP2 inhibits pore opening of the cyclic nucleotide-gated channel SthK.  |  Thon, O., et al. 2024. Nat Commun. 15: 8230. PMID: 39300080
10. Phosphatidylinositol 4,5-biphosphate stimulates parotid endoplasmic reticulum (Ca2+ + Mg2+)-ATP'ase.  |  Thiyagarajah, P. and Lim, SC. 1984. Biochem Int. 9: 625-30. PMID: 6151839
11. Significance of PIP2 hydrolysis and regulation of phospholipase C isozymes.  |  Lee, SB. and Rhee, SG. 1995. Curr Opin Cell Biol. 7: 183-9. PMID: 7612269
12. PIP2 and PIP as determinants for ATP inhibition of KATP channels.  |  Baukrowitz, T., et al. 1998. Science. 282: 1141-4. PMID: 9804555

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PIP2 Antibody (PIP2 2C11) has 36 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PIP2 Antibody (PIP2 2C11) Specifications

Alternate Product Name: Phosphatidylinositol 4,5-bisphosphate

Product Clone Name: PIP2 2C11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids:

Epitope Target: PIP2

Epitope Species: human

Product Reactivity/Detection: PIP2

Species Reactivity/Detection:

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IP, IF

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 36 citations in various scientific publications

Available Conjugates/Forms for PIP2 Antibody (PIP2 2C11): L: Azide-Free