Date published: 2025-10-11

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Pin1 Antibody (8C10): sc-81533

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Datasheets
  • Pin1 Antibody (8C10) is a mouse monoclonal IgG1, cited in 2 publications, provided at 50 µg/0.5 ml
  • raised against recombinant Pin1 of human origin
  • recommended for detection of Pin1 of mouse, rat, human and canine origin by WB and IP
  • See Pin1 (G-8): sc-46660 for Pin1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Pin1 Antibody (8C10). This work is in progress.

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Pin1 Antibody (8C10) is a mouse monoclonal IgG1 antibody engineered to recognize Pin1, also known as peptidylprolyl cis/trans isomerase, NIMA-interacting 1. Anti-Pin1 antibody (8C10) is raised against recombinant human Pin1 and targets a unique epitope, ensuring accurate and reliable detection. Pin1 is a pivotal peptidyl-prolyl isomerase located in both the nucleus and cytoplasm of cells, where Pin1 regulates the conformation and function of phosphorylated serine/threonine-proline motifs in various substrates. This regulation is essential for controlling critical cellular processes such as cell cycle progression, signal transduction, and protein stability, highlighting Pin1′s importance in maintaining cellular homeostasis and involvement in diseases like cancer and neurodegenerative disorders. Pin1 monoclonal antibody (8C10) demonstrates broad species reactivity, effectively detecting Pin1 from mouse, rat, human, and canine origins, and is validated for use in western blotting (WB) and immunoprecipitation (IP) applications. Anti-Pin1 antibody (8C10) serves as a valuable tool for researchers studying the mechanistic roles of Pin1 in cellular functions and disease mechanisms. Available in a non-conjugated form, Pin1 (8C10) antibody supports diverse experimental workflows, facilitating advanced investigations into the localization, expression, and interaction dynamics of Pin1 within different cellular contexts.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Pin1 Antibody (8C10)

sc-81533
50 µg/0.5 ml
$316.00

I am using Pin1 (8C10): sc-81533 antibody for immunoprecipitation, how much antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24

Hi, I wonder what the protein sequence of immunogen is?

Asked by: Yaooo
Thank you for your question. Pin1 Antibody (8C10): sc-81533 is a mouse monoclonal IgG1 raised against recombinant Pin1 of human origin. Unfortunately, the exact recombinant protein sequence used as an immunogen is not known.
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_10, d_10, h_6CST
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  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Robust signal seen in WB in Jurkat nuclear extractRobust signal seen in WB in Jurkat nuclear extract. -SCBT QC
Date published: 2015-05-23
Rated 5 out of 5 by from Strong band observed in WB in HeLaStrong band observed in WB in HeLa, K-562, Jurkat, NIH/3T3, PC-3 and Hep G2 nuclear extracts. -SCBT QC
Date published: 2015-02-25
Rated 5 out of 5 by from Antibody worked well for WB in serum starved AAntibody worked well for WB in serum starved A-431, A549, SKOV3, OVCAR-5, HaCaT, PC-3, HeLa and Hep G2 whole cell lysates. -SCBT QC
Date published: 2014-04-20
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Pin1 Antibody (8C10) is rated 5.0 out of 5 by 3.
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