Pim-1 CRISPR/Cas9 KO Plasmid (m): sc-422237
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pim-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Pim-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Pim-1 Antibody (G-11): sc-374116
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pim-1 CRISPR/Cas9 KO Plasmid (m) | sc-422237 | 20 µg | $397.00 |
Overview
Mouse Pim1 encodes Pim-1, a constitutively active serine/threonine kinase induced by cytokine and growth factor signaling and implicated in control of proliferation, survival, and metabolic adaptation. Pim-1 integrates with JAK/STAT and PI3K/AKT network outputs and modulates cell-cycle progression and apoptosis through phosphorylation of downstream effectors that influence transcription and protein stability. In hematopoietic and immune cell contexts, Pim-1 contributes to lymphocyte development and activation, and its dysregulation is frequently examined in models of oncogenic signaling, inflammation, and stress responses. Pim1 is also studied for roles in mitochondrial function and cellular senescence, providing mechanistic links between kinase signaling and tissue homeostasis.
Pim-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pim1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pim1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pim1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Pim-1 protein expression.
This CRISPR knockout system enables efficient generation of Pim1-deficient cell models for investigation of Pim-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pim1 exon(s) critical for Pim-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pim1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Pim-1 CRISPR/Cas9 KO Plasmid (m) and Pim-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pim1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Pim-1 HDR Plasmid (m) and Pim-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pim1 homology arms to support homology-directed repair at defined Pim1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.