Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PIG3 CRISPR/Cas9 KO Plasmid (h): sc-403356
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PIG3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PIG3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PIG3 Antibody (A-5): sc-166664
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PIG3 CRISPR/Cas9 KO Plasmid (h) | sc-403356 | 20 µg | $397.00 |
Overview
TP53I3 encodes PIG3, a p53-inducible oxidoreductase that contributes to cellular stress responses by modulating redox homeostasis and reactive oxygen species dynamics. PIG3 is linked to p53-dependent pathways controlling DNA damage signaling, apoptosis, and cell-cycle checkpoint regulation, and has been studied as a downstream effector influencing oxidative stress–driven phenotypes. Altered TP53I3/PIG3 expression has been associated with tumor biology and genotoxic stress sensitivity, making it relevant for mechanistic studies of p53 network integrity and redox-regulated signaling in human cells.
PIG3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TP53I3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TP53I3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TP53I3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PIG3 protein expression.
This CRISPR knockout system enables efficient generation of TP53I3-deficient cell models for investigation of PIG3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TP53I3 exon(s) critical for PIG3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TP53I3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PIG3 CRISPR/Cas9 KO Plasmid (h) and PIG3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TP53I3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PIG3 HDR Plasmid (h) and PIG3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TP53I3 homology arms to support homology-directed repair at defined TP53I3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.