PIG-K Antibody (G-6): sc-398611
- PIG-K Antibody (G-6) is a mouse monoclonal IgG1 λ PIG-K antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 96-395 mapping at the C-terminus of PIG-K of human origin
- PIG-K Antibody (G-6) is recommended for detection of PIG-K of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PIG-K Antibody (G-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for PIG-K Antibody (G-6) for WB applications. This reagent is now offered in a bundle with PIG-K Antibody (G-6) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
PIG-K Antibody (G-6) is a mouse monoclonal IgG1 antibody that detects PIG-K protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-PIG-K antibody (G-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PIG-K protein, also known as hGPI8, plays a critical role in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors, which are essential for the proper localization and function of many membrane proteins. Located in the endoplasmic reticulum, PIG-K serves as the catalytic subunit of the GPI transamidase complex, which recognizes the C-terminal signal sequences on proproteins, cleaves them, and replaces them with specific GPI lipids. This process is vital for the attachment of GPI anchors to proteins, influencing their stability and function within the cell membrane. PIG-K operates as a cysteine protease, featuring a Cis-His catalytic dyad that facilitates the cleavage of signal sequences and the formation of amide bonds between GPI and target proteins. PIG-K′s interaction with various components of the GPI biosynthetic pathway underscores its importance in cellular signaling and membrane dynamics, making PIG-K (G-6) monoclonal antibody an invaluable tool for researchers studying GPI-related processes in different species.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PIG-K Antibody (G-6) References:
- The glycosylphosphatidylinositol (GPI) signal sequence of human placental alkaline phosphatase is not recognized by human Gpi8p in the context of the yeast GPI anchoring machinery. | Meyer, U., et al. 2002. Mol Microbiol. 46: 745-8. PMID: 12410831
- Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post-translational transfer to proteins. | Eisenhaber, B., et al. 2003. Bioessays. 25: 367-85. PMID: 12655644
- GPI transamidase of Trypanosoma brucei has two previously uncharacterized (trypanosomatid transamidase 1 and 2) and three common subunits. | Nagamune, K., et al. 2003. Proc Natl Acad Sci U S A. 100: 10682-7. PMID: 12958211
- Assignment of phosphatidylinositol glycan, class K (PIGK) gene to porcine chromosome 6q32 by somatic cell and radiation hybrid panel mapping. | Park, EW., et al. 2005. Cytogenet Genome Res. 108: 363. PMID: 15628035
- Recent developments in the molecular, biochemical and functional characterization of GPI8 and the GPI-anchoring mechanism [review]. | Zacks, MA. and Garg, N. 2006. Mol Membr Biol. 23: 209-25. PMID: 16785205
- Biosynthesis and function of GPI proteins in the yeast Saccharomyces cerevisiae. | Pittet, M. and Conzelmann, A. 2007. Biochim Biophys Acta. 1771: 405-20. PMID: 16859984
- Processing of nascent proteins to glycosylphosphatidylinositol-anchored forms in cell-free systems. | Kodukula, K., et al. 1995. Methods Enzymol. 250: 536-47. PMID: 7651176
- COOH-terminal processing of nascent polypeptides by the glycosylphosphatidylinositol transamidase in the presence of hydrazine is governed by the same parameters as glycosylphosphatidylinositol addition. | Ramalingam, S., et al. 1996. Proc Natl Acad Sci U S A. 93: 7528-33. PMID: 8755508
- Yeast Gpi8p is essential for GPI anchor attachment onto proteins. | Benghezal, M., et al. 1996. EMBO J. 15: 6575-83. PMID: 8978684
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PIG-K Antibody (G-6) | sc-398611 | 200 µg/ml | $322.00 | |||
PIG-K Antibody (G-6): m-IgGλ BP-HRP Bundle | sc-524422 | 200 µg Ab, 40 µg BP | $361.00 | |||
PIG-K Antibody (G-6) AC | sc-398611 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PIG-K Antibody (G-6) HRP | sc-398611 HRP | 200 µg/ml | $322.00 | |||
PIG-K Antibody (G-6) FITC | sc-398611 FITC | 200 µg/ml | $336.00 | |||
PIG-K Antibody (G-6) PE | sc-398611 PE | 200 µg/ml | $349.00 | |||
PIG-K Antibody (G-6) Alexa Fluor® 488 | sc-398611 AF488 | 200 µg/ml | $364.00 | |||
PIG-K Antibody (G-6) Alexa Fluor® 546 | sc-398611 AF546 | 200 µg/ml | $364.00 | |||
PIG-K Antibody (G-6) Alexa Fluor® 594 | sc-398611 AF594 | 200 µg/ml | $364.00 | |||
PIG-K Antibody (G-6) Alexa Fluor® 647 | sc-398611 AF647 | 200 µg/ml | $364.00 | |||
PIG-K Antibody (G-6) Alexa Fluor® 680 | sc-398611 AF680 | 200 µg/ml | $364.00 | |||
PIG-K Antibody (G-6) Alexa Fluor® 790 | sc-398611 AF790 | 200 µg/ml | $364.00 |