PIF1 Antibody (F-10): sc-48377

PIF1 Antibody (F-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects PIF1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PIF1 (F-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PIF1 functions as a single-stranded DNA-dependent ATPase and helicase, unwinding DNA in a 5′ to 3′ direction, which is essential for DNA replication and telomere length maintenance in organisms such as Saccharomyces cerevisiae. This function helps prevent genomic instability, which can lead to various diseases, including cancer. PIF1 is highly conserved across species, and mutations can result in telomere elongation, indicating a role as a catalytic inhibitor of both new telomere formation and elongation. In humans, PIF1 preferentially binds to telomeric DNA, where PIF1 inhibits telomerase activity, thereby playing a significant role in cellular aging and tumor suppression. PIF1 counteracts the activity of the RecQ homolog Sgs1 helicase, and PIF1 degradation is regulated by the ubiquitin-26S proteasome pathway, highlighting an importance in maintaining genomic integrity.

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PIF1 Antibody (F-10) References:

1. Schizosaccharomyces pombe pfh1+ encodes an essential 5' to 3' DNA helicase that is a member of the PIF1 subfamily of DNA helicases.  |  Zhou, JQ., et al. 2002. Mol Biol Cell. 13: 2180-91. PMID: 12058079
2. The human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity.  |  Zhang, DH., et al. 2006. Nucleic Acids Res. 34: 1393-404. PMID: 16522649
3. Evidence suggesting that Pif1 helicase functions in DNA replication with the Dna2 helicase/nuclease and DNA polymerase delta.  |  Budd, ME., et al. 2006. Mol Cell Biol. 26: 2490-500. PMID: 16537895
4. Characterization of ATPase activity of recombinant human Pif1.  |  Huang, Y., et al. 2006. Acta Biochim Biophys Sin (Shanghai). 38: 335-41. PMID: 16680374
5. The absence of Top3 reveals an interaction between the Sgs1 and Pif1 DNA helicases in Saccharomyces cerevisiae.  |  Wagner, M., et al. 2006. Genetics. 174: 555-73. PMID: 16816432
6. Roles of Pif1-like helicases in the maintenance of genomic stability.  |  Boulé, JB. and Zakian, VA. 2006. Nucleic Acids Res. 34: 4147-53. PMID: 16935874
7. PIF1: a DNA helicase in yeast mitochondria.  |  Lahaye, A., et al. 1991. EMBO J. 10: 997-1007. PMID: 1849081
8. Dynamic regulation of Pif1 acetylation is crucial to the maintenance of genome stability.  |  Ononye, OE., et al. 2021. Curr Genet. 67: 85-92. PMID: 33079209
9. Role and Regulation of Pif1 Family Helicases at the Replication Fork.  |  Malone, EG., et al. 2022. Int J Mol Sci. 23: PMID: 35409096
10. Phytochromes enhance SOS2-mediated PIF1 and PIF3 phosphorylation and degradation to promote Arabidopsis salt tolerance.  |  Ma, L., et al. 2023. Plant Cell. 35: 2997-3020. PMID: 37119239
11. PIF1 DNA helicase from Saccharomyces cerevisiae. Biochemical characterization of the enzyme.  |  Lahaye, A., et al. 1993. J Biol Chem. 268: 26155-61. PMID: 8253734
12. The saccharomyces PIF1 DNA helicase inhibits telomere elongation and de novo telomere formation.  |  Schulz, VP. and Zakian, VA. 1994. Cell. 76: 145-55. PMID: 8287473

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PIF1 Antibody (F-10) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-PIF1 Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PIF1 Antibody (F-10) Specifications

Alternate Product Name: PIF1

Product Clone Name: F-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: PIF1

Epitope Species: mouse

Product Reactivity/Detection: PIF1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for PIF1 Antibody (F-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent