PIBF Antibody (F-12): sc-166904

PIBF Antibody (F-12) is a mouse monoclonal IgG1 antibody that detects Progesterone-Induced Blocking Factor 1 (PIBF) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PIBF plays a crucial role during pregnancy, as PIBF is synthesized in response to progesterone by progesterone receptor-positive T lymphocytes, predominantly gamma-delta T cells. PIBF is essential for modulating the immune response, particularly by inhibiting the activity of natural killer (NK) cells, which prevents the release of perforin and subsequent lysis of target cells. Notably, PIBF expression is significantly elevated in healthy pregnant women compared to those at risk for premature pregnancy termination, highlighting PIBF′s importance in maintaining pregnancy. PIBF is primarily located in the nucleus in its full-length form, where PIBF is believed to function as a transcription factor, while shorter forms, which are secreted upon cell activation, may act as cytokines. The PIBF gene encodes a hydrophilic protein consisting of 757 amino acids, featuring an N-terminal signal sequence, a leucine zipper motif, a basic zipper sequence, a PEST sequence, a nuclear localization signal, and an endoplasmic reticulum membrane retention signal, along with numerous presumed N-glycosylation and phosphorylation sites that may influence PIBF function and interactions within the cellular environment. Anti-PIBF antibody (F-12) is an invaluable tool for researchers investigating PIBF′s role in pregnancy and immune regulation.

The preferred PIBF antibody is PIBF Antibody (A-2): sc-376840.