PIBF Antibody (A-2): sc-376840

PIBF Antibody (A-2) is a mouse monoclonal IgM antibody that detects PIBF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PIBF (A-2) antibody is available as the non-conjugated form. Progesterone-Induced Blocking Factor 1 (PIBF) plays a crucial role during pregnancy by modulating immune responses. PIBF is synthesized in response to progesterone by progesterone receptor-positive T lymphocytes, predominantly gamma-delta T cells. PIBF is essential for maintaining a healthy pregnancy, as PIBF inhibits the cytotoxic activity of natural killer (NK) cells, preventing them from lysing fetal cells. Notably, PIBF expression is significantly elevated in healthy pregnant women compared to those at risk for premature pregnancy termination, highlighting PIBF importance in pregnancy maintenance. Structurally, PIBF gene encodes a hydrophilic protein consisting of 757 amino acids, featuring an N-terminal signal sequence, a leucine zipper motif, a basic zipper sequence, a PEST sequence, a nuclear localization signal, and an endoplasmic reticulum membrane retention signal, along with numerous presumed N-glycosylation and phosphorylation sites. These structural elements are vital for PIBF function, as these elements facilitate proper localization and interaction with other cellular components, thereby influencing PIBF role as a transcription factor in its full-length form and potentially as a cytokine in its shorter forms.

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PIBF Antibody (A-2) References:

1. Progesterone induced blocking factor (PIBF) mediates progesterone induced suppression of decidual lymphocyte cytotoxicity.  |  Laskarin, G., et al. 2002. Am J Reprod Immunol. 48: 201-9. PMID: 12516630
2. Molecular cloning and immunologic characterization of a novel cDNA coding for progesterone-induced blocking factor.  |  Polgar, B., et al. 2003. J Immunol. 171: 5956-63. PMID: 14634107
3. PIBF (progesterone induced blocking factor) is overexpressed in highly proliferating cells and associated with the centrosome.  |  Lachmann, M., et al. 2004. Int J Cancer. 112: 51-60. PMID: 15305375
4. The impact of dydrogesterone supplementation on hormonal profile and progesterone-induced blocking factor concentrations in women with threatened abortion.  |  Kalinka, J. and Szekeres-Bartho, J. 2005. Am J Reprod Immunol. 53: 166-71. PMID: 15760377
5. Miscarriage in the first trimester according to the presence or absence of the progesterone-induced blocking factor at three to five weeks from conception in progesterone supplemented women.  |  Check, JH., et al. 2005. Clin Exp Obstet Gynecol. 32: 13-4. PMID: 15864927
6. Lymphocyte immunotherapy (LI) increases serum levels of progesterone induced blocking factor (PIBF).  |  Check, JH., et al. 1997. Am J Reprod Immunol. 37: 17-20. PMID: 9138449
7. Expression of an immunomodulatory protein known as progesterone induced blocking factor (PIBF) does not correlate with first trimester spontaneous abortions in progesterone supplemented women.  |  Check, JH., et al. 1997. Am J Reprod Immunol. 37: 330-4. PMID: 9161642

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PIBF Antibody (A-2) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PIBF Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PIBF Antibody (A-2) Specifications

Alternate Product Name: PIBF

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 16-45

Epitope Target: PIBF

Epitope Species: human

Product Reactivity/Detection: PIBF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PIBF Antibody (A-2): P: Blocking Peptide