Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PI 3-kinase p85β Double Nickase Plasmid (h): sc-401991-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PI 3-kinase p85β Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PI 3-kinase p85β Double Nickase Plasmid (h) and PI 3-kinase p85β Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PIK3R2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PI 3-kinase p85β Antibody (H-1): sc-515646
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PI 3-kinase p85β Double Nickase Plasmid (h) | sc-401991-NIC | 20 µg | $410.00 | |||
PI 3-kinase p85β Double Nickase Plasmid (h2) | sc-401991-NIC-2 | 20 µg | $410.00 |
PIK3R2 encodes the p85β regulatory subunit of class IA phosphoinositide 3-kinase (PI3K), which stabilizes and modulates p110 catalytic isoforms and couples activated receptor tyrosine kinases and adaptor proteins to downstream PI3K–AKT–mTOR signaling. Through control of PIP3 generation, PI 3-kinase p85β influences cell growth, survival, metabolism, cytoskeletal dynamics, and vesicular trafficking, integrating cues from insulin/IGF and growth factor pathways. Altered PI3K pathway regulation is widely implicated in oncogenic signaling, metabolic dysregulation, and immune cell function, making PIK3R2 a relevant node for dissecting pathway wiring and feedback. In research settings, perturbing PIK3R2 supports mechanistic studies of PI3K isoform usage, signal amplitude/termination, and pathway cross-talk with MAPK and mTOR complexes.
PI 3-kinase p85β Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PIK3R2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PIK3R2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PIK3R2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PIK3R2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.