Phox2b Antibody (B-11): sc-376997

Phox2b Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Phox2b protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Phox2b (B-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Phox2b plays a critical role in neuronal differentiation, particularly within developing sympathetic, parasympathetic, and enteric ganglia, as Phox2b is essential for differentiation of all central and nonperipheral noradrenergic centers in brain. Phox2b is primarily located in nucleus of neuronal cells, where Phox2b functions as a transcription factor, regulating expression of genes necessary for neuronal development. Nuclear localization of Phox2b enables direct influence on gene transcription, thereby guiding fate of neural crest-derived cells as they differentiate into various neuronal lineages. Phox2b is crucial for regulation of endogenous tyrosine hydroxylase and dopamine-beta hydroxylase, which are vital for catecholamine synthesis. Interaction between Phox2b and other transcription factors, such as Phox2a, underscores Phox2b′s significance in broader context of neurogenesis and establishment of enteric nervous system.

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Phox2b Antibody (B-11) References:

1. Specification of neurotransmitter identity by Phox2 proteins in neural crest stem cells.  |  Lo, L., et al. 1999. Neuron. 22: 693-705. PMID: 10230790
2. The homeobox gene Phox2b is essential for the development of autonomic neural crest derivatives.  |  Pattyn, A., et al. 1999. Nature. 399: 366-70. PMID: 10360575
3. The Phox2 homeodomain proteins are sufficient to promote the development of sympathetic neurons.  |  Stanke, M., et al. 1999. Development. 126: 4087-94. PMID: 10457017
4. Expression of Ret-, p75(NTR)-, Phox2a-, Phox2b-, and tyrosine hydroxylase-immunoreactivity by undifferentiated neural crest-derived cells and different classes of enteric neurons in the embryonic mouse gut.  |  Young, HM., et al. 1999. Dev Dyn. 216: 137-52. PMID: 10536054
5. Specification of the central noradrenergic phenotype by the homeobox gene Phox2b.  |  Pattyn, A., et al. 2000. Mol Cell Neurosci. 15: 235-43. PMID: 10736201
6. Germline mutations of the paired-like homeobox 2B (PHOX2B) gene in neuroblastoma.  |  Bourdeaut, F., et al. 2005. Cancer Lett. 228: 51-8. PMID: 15949893
7. Chemosensitive Phox2b-expressing neurons are crucial for hypercapnic ventilatory response in the nucleus tractus solitarius.  |  Fu, C., et al. 2017. J Physiol. 595: 4973-4989. PMID: 28488367
8. Phox2b Immunohistochemical Staining in Detecting Enteric Neural Crest Cells in Hirschsprung Disease.  |  Alturkustani, M., et al. 2021. Pediatr Dev Pathol. 24: 19-26. PMID: 32975470
9. Reduced PHOX2B stability causes axonal growth impairment in motor neurons with TARDBP mutations.  |  Mitsuzawa, S., et al. 2021. Stem Cell Reports. 16: 1527-1541. PMID: 34048688
10. Disordered breathing in a Pitt-Hopkins syndrome model involves Phox2b-expressing parafacial neurons and aberrant Nav1.8 expression.  |  Cleary, CM., et al. 2021. Nat Commun. 12: 5962. PMID: 34645823
11. Intrinsic properties and synaptic connectivity of Phox2b-expressing neurons in rat rostral parvocellular reticular formation.  |  Kajiwara, R., et al. 2022. Neurosci Res. 178: 41-51. PMID: 34973291
12. Mapping of the ARIX homeodomain gene to mouse chromosome 7 and human chromosome 11q13.  |  Johnson, KR., et al. 1996. Genomics. 33: 527-31. PMID: 8661014

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Phox2b Antibody (B-11) has 41 citations in a variety of scientific publications.

Primary antibodies like Anti-Phox2b Antibody (B-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Phox2b Antibody (B-11) Specifications

Alternate Product Name: PHOX2B Homeodomain Protein

Product Clone Name: B-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 11-70

Epitope Target: Phox2b

Epitope Species: human

Product Reactivity/Detection: Phox2b

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 41 citations in various scientific publications

Available Conjugates/Forms for Phox2b Antibody (B-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent