PHOSPHO1 Antibody (II-91) recommended for detection of PHOSPHO1 of mouse, rat and human origin by WB, IP and ELISA

PHOSPHO1 Antibody (II-91): sc-100351

PHOSPHO1 Antibody (II-91) is rated 4.0 out of 5 by 2.
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5
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4
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2
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Datasheets

    • Anti-PHOSPHO1 Antibody (II-91) is a mouse monoclonal IgG1 (kappa light chain) PHOSPHO1 antibody provided at 100 µg/ml
    • raised against recombinant PHOSPHO1 of human origin
    • recommended for detection of PHOSPHO1 of mouse, rat and human origin by WB, IP and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PHOSPHO1 (II-91): sc-100351.

For Western Blot, is it recommended to use denatured or non-denatured conditions with PHOSPHO1 (II-91): sc-100351 antibody?

Asked by: Professor Griffin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from 2 band for WB I saw 2 band in your datasheets. Also in my mouse sample i saw very close 2 band for phospho1. Which band is the right one? Why there have 2 band?
Date published: 2019-04-09
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