PHOSPHO1 Antibody (II-91): sc-100351

PHOSPHO1 Antibody (II-91) is a mouse monoclonal IgG1 kappa light chain antibody that detects PHOSPHO1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-PHOSPHO1 antibody (II-91) is available as the non-conjugated format. PHOSPHO1, also known as phosphatase, orphan 1, is a 267 amino acid enzyme belonging to the haloacid dehalogenase superfamily of magnesium-dependent hydrolases. PHOSPHO1 is predominantly expressed in bone and cartilage, where PHOSPHO1 is localized to the osteoid layer of the periosteum, a critical area for bone formation. PHOSPHO1 plays a key role in the mineralization process during skeletal development by cleaving phosphoethanolamine and phosphocholine to release inorganic phosphate, which is essential for the calcification of the extracellular matrix. PHOSPHO1 is restricted to sites of mineralization, and inhibition of PHOSPHO1 has been shown to impair the ability of matrix vesicles to facilitate calcification in bone, highlighting its crucial function in skeletal integrity. PHOSPHO1 features three conserved catalytic motifs that are characteristic of the haloacid dehalogenase superfamily, underscoring its evolutionary significance and functional capabilities in phosphate metabolism.

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PHOSPHO1 Antibody (II-91) References:

1. Chromosomal localization of the chicken and mammalian orthologues of the orphan phosphatase PHOSPHO1 gene.  |  Houston, B., et al. 2002. Anim Genet. 33: 451-4. PMID: 12464021
2. Comparative modelling of human PHOSPHO1 reveals a new group of phosphatases within the haloacid dehalogenase superfamily.  |  Stewart, AJ., et al. 2003. Protein Eng. 16: 889-95. PMID: 14983068
3. PHOSPHO1-A novel phosphatase specifically expressed at sites of mineralisation in bone and cartilage.  |  Houston, B., et al. 2004. Bone. 34: 629-37. PMID: 15050893
4. Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities.  |  Roberts, SJ., et al. 2004. Biochem J. 382: 59-65. PMID: 15175005
5. Probing the substrate specificities of human PHOSPHO1 and PHOSPHO2.  |  Roberts, SJ., et al. 2005. Biochim Biophys Acta. 1752: 73-82. PMID: 16054448
6. The presence of PHOSPHO1 in matrix vesicles and its developmental expression prior to skeletal mineralization.  |  Stewart, AJ., et al. 2006. Bone. 39: 1000-1007. PMID: 16837257
7. Functional involvement of PHOSPHO1 in matrix vesicle-mediated skeletal mineralization.  |  Roberts, S., et al. 2007. J Bone Miner Res. 22: 617-27. PMID: 17227223
8. PHOSPHO1 is a skeletal regulator of insulin resistance and obesity.  |  Suchacki, KJ., et al. 2020. BMC Biol. 18: 149. PMID: 33092598
9. Increased PHOSPHO1 expression mediates cortical bone mineral density in renal osteodystrophy.  |  Hsu, SN., et al. 2022. J Endocrinol. 254: 153-167. PMID: 35900032
10. The emerging roles of PHOSPHO1 and its regulated phospholipid homeostasis in metabolic disorders.  |  Liu, Y., et al. 2022. Front Physiol. 13: 935195. PMID: 35957983
11. Increased PHOSPHO1 and alkaline phosphatase expression during the anabolic bone response to intermittent parathyroid hormone delivery.  |  Houston, DA., et al. 2023. Cell Biochem Funct. 41: 189-201. PMID: 36540015
12. Orphan Nuclear Receptor NR4A3 Promotes Vascular Calcification via Histone Lactylation.  |  Ma, W., et al. 2024. Circ Res. 134: 1427-1447. PMID: 38629274

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PHOSPHO1 Antibody (II-91) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-PHOSPHO1 Antibody (II-91) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PHOSPHO1 Antibody (II-91) Specifications

Alternate Product Name: PHOSPHO1

Product Clone Name: II-91

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 168-267

Epitope Target: PHOSPHO1

Epitope Species: human

Product Reactivity/Detection: PHOSPHO1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for PHOSPHO1 Antibody (II-91): N/A