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Phosphate ABC transporter substrate-binding protein Antibody (A-10): sc-398074

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  • Phosphate ABC transporter substrate-binding protein Antibody (A-10) is a mouse monoclonal IgG1 κ Phosphate ABC transporter substrate-binding protein antibody provided at 200 µg/ml
  • raised against amino acids 1-288 representing full length Phosphate ABC transporter substrate-binding protein of Streptococcus equi subsp. equi 4047 origin
  • Phosphate ABC transporter substrate-binding protein Antibody (A-10) is recommended for detection of Phosphate ABC transporter substrate-binding protein of Streptococcus equi subsp. equi 4047 origin by WB, IP, IF and ELISA
  • Anti-Phosphate ABC transporter substrate-binding protein Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Phosphate ABC transporter substrate-binding protein Antibody (A-10) for WB applications. These reagents are now offered in bundles with Phosphate ABC transporter substrate-binding protein Antibody (A-10) (see ordering information below).
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    Phosphate ABC transporter substrate-binding protein Antibody (A-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Phosphate ABC transporter substrate-binding protein of Streptococcus equi subsp. equi 4047 origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Phosphate ABC transporter substrate-binding protein antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Phosphate ABC transporter substrate-binding protein plays a crucial role in phosphate transport across bacterial cell membranes, essential for Streptococcus equi survival and growth. This transport mechanism facilitates phosphate uptake, supporting vital cellular processes like energy metabolism and nucleic acid synthesis. S. equi′s efficient phosphate transport capability enhances pathogenicity by enabling bacterial survival in nutrient-limited host environments. Understanding Phosphate ABC transporter substrate-binding protein function provides valuable insights into potential therapeutic targets for treating strangles, a highly contagious equine disease characterized by severe respiratory distress and lymphadenitis.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Phosphate ABC transporter substrate-binding protein Antibody (A-10) References:

    1. Getting to grips with strangles: an effective multi-component recombinant vaccine for the protection of horses from Streptococcus equi infection.  |  Guss, B., et al. 2009. PLoS Pathog. 5: e1000584. PMID: 19763180
    2. Molecular characterisation of 'strangles' outbreaks in the UK: the use of M-protein typing of Streptococcus equi ssp. equi.  |  Ivens, PA., et al. 2011. Equine Vet J. 43: 359-64. PMID: 21492215
    3. Association of Streptococcus equi with equine monocytes.  |  Mérant, C., et al. 2011. Vet Immunol Immunopathol. 143: 83-6. PMID: 21752476
    4. Streptococcus equi: a pathogen restricted to one host.  |  Waller, AS., et al. 2011. J Med Microbiol. 60: 1231-1240. PMID: 21757503
    5. Streptococcus equi subspecies equi infection (strangles) in horses.  |  Boyle, A. 2011. Compend Contin Educ Vet. 33: E1-7; quiz E8. PMID: 21870348
    6. Antiphagocytic function of an IgG glycosyl hydrolase from Streptococcus equi subsp. equi and its use as a vaccine component.  |  Flock, M., et al. 2012. Infect Immun. 80: 2914-9. PMID: 22615244
    7. Development of a novel mucosal vaccine against strangles by supercritical enhanced atomization spray-drying of Streptococcus equi extracts and evaluation in a mouse model.  |  Rodrigues, MA., et al. 2012. Eur J Pharm Biopharm. 82: 392-400. PMID: 22841882
    8. Detection of Streptococcus equi subspecies equi using a triplex qPCR assay.  |  Webb, K., et al. 2013. Vet J. 195: 300-4. PMID: 22884566

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Phosphate ABC transporter substrate-binding protein Antibody (A-10)

    sc-398074
    200 µg/ml
    $322.00

    Phosphate ABC transporter substrate-binding protein Antibody (A-10): m-IgG Fc BP-HRP Bundle

    sc-527735
    200 µg Ab; 10 µg BP
    $361.00

    Phosphate ABC transporter substrate-binding protein Antibody (A-10): m-IgG1 BP-HRP Bundle

    sc-533108
    200 µg Ab; 20 µg BP
    $361.00

    Phosphate ABC transporter substrate-binding protein Antibody (A-10) AC

    sc-398074 AC
    500 µg/ml, 25% agarose
    $424.00

    Phosphate ABC transporter substrate-binding protein Antibody (A-10) HRP

    sc-398074 HRP
    200 µg/ml
    $322.00

    Phosphate ABC transporter substrate-binding protein Antibody (A-10) FITC

    sc-398074 FITC
    200 µg/ml
    $336.00

    Phosphate ABC transporter substrate-binding protein Antibody (A-10) PE

    sc-398074 PE
    200 µg/ml
    $349.00

    Phosphate ABC transporter substrate-binding protein Antibody (A-10) Alexa Fluor® 488

    sc-398074 AF488
    200 µg/ml
    $364.00

    Phosphate ABC transporter substrate-binding protein Antibody (A-10) Alexa Fluor® 546

    sc-398074 AF546
    200 µg/ml
    $364.00

    Phosphate ABC transporter substrate-binding protein Antibody (A-10) Alexa Fluor® 594

    sc-398074 AF594
    200 µg/ml
    $364.00

    Phosphate ABC transporter substrate-binding protein Antibody (A-10) Alexa Fluor® 647

    sc-398074 AF647
    200 µg/ml
    $364.00

    Phosphate ABC transporter substrate-binding protein Antibody (A-10) Alexa Fluor® 680

    sc-398074 AF680
    200 µg/ml
    $364.00

    Phosphate ABC transporter substrate-binding protein Antibody (A-10) Alexa Fluor® 790

    sc-398074 AF790
    200 µg/ml
    $364.00

    How much sample do you recommend loading on the gel for Western blot detection using Phosphate ABC transporter substrate-binding protein (A-10): sc-398074?

    Asked by: Professor Griffin
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2023-09-01
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    Rated 5 out of 5 by from Very good Western blot data of PhosphateVery good Western blot data of Phosphate ABC transporter substrate-binding protein expression in Streptococcus equi (virulent), Streptococcus equi (avirulent), Rhodococcus equi and Escherichia coli whole cell lysates. -SCBT QC
    Date published: 2014-08-11
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    Phosphate ABC transporter substrate-binding protein Antibody (A-10) is rated 5.0 out of 5 by 1.
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