PhLP Antibody (A-8): sc-376918
- PhLP Antibody (A-8) is a mouse monoclonal IgG1 κ PhLP antibody provided at 200 µg/ml
- raised against amino acids 66-175 mapping within an internal region of PhLP of human origin
- PhLP Antibody (A-8) is recommended for detection of PhLP of human origin by WB, IP, IF and ELISA
- Anti-PhLP Antibody (A-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PhLP Antibody (A-8) for WB applications. These reagents are now offered in bundles with PhLP Antibody (A-8) (see ordering information below).
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PhLP Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects PhLP protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-PhLP antibody (A-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Phosducin-like protein (PhLP, PDCL) plays a crucial role as an ethanol-responsive modulator of heterotrimeric G proteins, which are essential for transmitting signals from various stimuli outside the cell to its interior. PhLP is primarily located in the cytoplasm and interacts with the beta-gamma subunits of G proteins, thereby influencing G-protein signaling pathways. Notably, PhLP shares significant amino acid sequence homology with phosducin (Phd), a phosphoprotein predominantly expressed in the retina and pineal gland, and both proteins are involved in the regulation of G-protein signaling. PhLP interacts with G beta-gamma through a short C-terminal binding site, which is also the site for phosphorylation by G protein-coupled receptor kinase 2 (GRK2) between residues 195 and 218. This phosphorylation is vital for modulating PhLP activity, thereby affecting its role in calcium-evoked exocytosis in adrenal medullary chromaffin cells. Additionally, glycosylated forms of PhLP have been shown to regulate opioid receptor function in the mouse brain, highlighting PhLP importance in neuropharmacology and cellular signaling.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PhLP Antibody (A-8) References:
- Cloning and characterization of the rat and human phosducin-like protein genes: structure, expression and chromosomal localization. | Thibault, C., et al. 1999. Biochim Biophys Acta. 1444: 346-54. PMID: 10095058
- Phosphorylation of phosducin and phosducin-like protein by G protein-coupled receptor kinase 2. | Ruiz-Gómez, A., et al. 2000. J Biol Chem. 275: 29724-30. PMID: 10884381
- Regulation of exocytosis in chromaffin cells by phosducin-like protein, a protein interacting with G protein betagamma subunits. | Gensse, M., et al. 2000. FEBS Lett. 480: 184-8. PMID: 11034325
- Glycosylated phosducin-like protein long regulates opioid receptor function in mouse brain. | Garzón, J., et al. 2002. Neuropharmacology. 42: 813-28. PMID: 12015208
- Phosducin-like protein: an ethanol-responsive potential modulator of guanine nucleotide-binding protein function. | Miles, MF., et al. 1993. Proc Natl Acad Sci U S A. 90: 10831-5. PMID: 8248177
- Identification of a C-terminal binding site for G-protein betagamma-subunits in phosducin-like protein. | Schröder, S., et al. 1997. FEBS Lett. 401: 243-6. PMID: 9013896
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PhLP Antibody (A-8) | sc-376918 | 200 µg/ml | $322.00 | |||
PhLP Antibody (A-8): m-IgG Fc BP-HRP Bundle | sc-527552 | 200 µg Ab; 10 µg BP | $361.00 | |||
PhLP Antibody (A-8): m-IgG1 BP-HRP Bundle | sc-532925 | 200 µg Ab; 20 µg BP | $361.00 | |||
PhLP Antibody (A-8) AC | sc-376918 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PhLP Antibody (A-8) HRP | sc-376918 HRP | 200 µg/ml | $322.00 | |||
PhLP Antibody (A-8) FITC | sc-376918 FITC | 200 µg/ml | $336.00 | |||
PhLP Antibody (A-8) PE | sc-376918 PE | 200 µg/ml | $349.00 | |||
PhLP Antibody (A-8) Alexa Fluor® 488 | sc-376918 AF488 | 200 µg/ml | $364.00 | |||
PhLP Antibody (A-8) Alexa Fluor® 546 | sc-376918 AF546 | 200 µg/ml | $364.00 | |||
PhLP Antibody (A-8) Alexa Fluor® 594 | sc-376918 AF594 | 200 µg/ml | $364.00 | |||
PhLP Antibody (A-8) Alexa Fluor® 647 | sc-376918 AF647 | 200 µg/ml | $364.00 | |||
PhLP Antibody (A-8) Alexa Fluor® 680 | sc-376918 AF680 | 200 µg/ml | $364.00 | |||
PhLP Antibody (A-8) Alexa Fluor® 790 | sc-376918 AF790 | 200 µg/ml | $364.00 |