PHKG1 Antibody (RR-34): sc-100536

PHKG1 Antibody (RR-34) is a mouse monoclonal IgG2a kappa light chain antibody that detects PHKG1 protein of human origin by immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-PHKG1 antibody (RR-34) is available as a non-conjugated format. PHKG1, or Phosphorylase kinase subunit γ1, is a crucial component of phosphorylase kinase (PHK), which is a hexadecameric protein composed of four α chains, four β chains, four γ chains, and four δ chains. The γ chains serve as catalytic units, while α and β chains function as regulatory components, and δ chains are calmodulins that mediate calcium signaling. PHKG1 contains two calmodulin-binding domains and one protein kinase domain, which are essential for enzymatic activity. As the catalytic chain of PHK, PHKG1 plays a vital role in the glycogenolytic pathway by catalyzing the phosphorylation and activation of glycogen phosphorylase, thereby facilitating glucose mobilization from glycogen stores. This function is particularly important in maintaining glucose homeostasis during fasting or intense physical activity. Mutations in the gene encoding PHKG1 can lead to phosphorylase kinase deficiency, resulting in glycogen storage disease type 9C (GSD9C), a condition characterized by glycogen accumulation in the liver and associated metabolic disturbances.

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PHKG1 Antibody (RR-34) References:

1. Phosphorylase kinase deficient liver glycogenosis: progression to cirrhosis in infancy associated with PHKG2 mutations (H144Y and L225R).  |  Burwinkel, B., et al. 2000. J Med Genet. 37: 376-7. PMID: 10905889
2. Severe phenotype of phosphorylase kinase-deficient liver glycogenosis with mutations in the PHKG2 gene.  |  Burwinkel, B., et al. 2003. Pediatr Res. 54: 834-9. PMID: 12930917
3. Molecular cloning and enzymatic analysis of the rat homolog of 'PhK-gamma T,' an isoform of phosphorylase kinase catalytic subunit.  |  Calalb, MB., et al. 1992. J Biol Chem. 267: 1455-63. PMID: 1370475
4. Effects of Scutellariae Radix on gene expression in HEK 293 cells using cDNA microarray.  |  Chen, CS., et al. 2006. J Ethnopharmacol. 105: 346-51. PMID: 16406416
5. Glycogen storage disease type IX: High variability in clinical phenotype.  |  Beauchamp, NJ., et al. 2007. Mol Genet Metab. 92: 88-99. PMID: 17689125
6. Messenger ribonucleic acid encoding an apparent isoform of phosphorylase kinase catalytic subunit is abundant in the adult testis.  |  Hanks, SK. 1989. Mol Endocrinol. 3: 110-6. PMID: 2915644
7. Lung adenocarcinoma-derived vWF promotes tumor metastasis by regulating PHKG1-mediated glycogen metabolism.  |  Gu, J., et al. 2022. Cancer Sci. 113: 1362-1376. PMID: 35150045
8. Five genes identified as prognostic markers for colorectal cancer through the integration of genome-wide association study and expression quantitative trait loci data.  |  Zhang, C., et al. 2024. Per Med. 21: 103-116. PMID: 38380524
9. Human cDNA encoding the muscle isoform of the phosphorylase kinase gamma subunit (PHKG1).  |  Wehner, M. and Kilimann, MW. 1995. Hum Genet. 96: 616-8. PMID: 8530014
10. The testis isoform of the phosphorylase kinase catalytic subunit (PhK-gammaT) plays a critical role in regulation of glycogen mobilization in developing lung.  |  Liu, L., et al. 1996. J Biol Chem. 271: 11761-6. PMID: 8662648
11. Mutations in the testis/liver isoform of the phosphorylase kinase gamma subunit (PHKG2) cause autosomal liver glycogenosis in the gsd rat and in humans.  |  Maichele, AJ., et al. 1996. Nat Genet. 14: 337-40. PMID: 8896567
12. Liver glycogenosis due to phosphorylase kinase deficiency: PHKG2 gene structure and mutations associated with cirrhosis.  |  Burwinkel, B., et al. 1998. Hum Mol Genet. 7: 149-54. PMID: 9384616

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PHKG1 Antibody (RR-34) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PHKG1 Antibody (RR-34) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PHKG1 Antibody (RR-34) Specifications

Alternate Product Name: PHKG1

Product Clone Name: RR-34

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-388

Epitope Target: PHKG1

Epitope Species: human

Product Reactivity/Detection: PHKG1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, IHC(P), ELISA

Vialing Information: Each vial contains 50 µg IgG_2a kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PHKG1 Antibody (RR-34): N/A