PHF5A CRISPR/Cas9 KO Plasmid (h): sc-407986
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PHF5A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PHF5A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PHF5A CRISPR/Cas9 KO Plasmid (h) | sc-407986 | 20 µg | $397.00 |
Overview
PHF5A (PHD finger protein 5A) is an essential component of the SF3b subcomplex within the U2 snRNP, where it supports recognition of branch point sequences and helps define splice site choice during pre-mRNA splicing. Through its role in spliceosome assembly and spliceosomal dynamics, PHF5A influences transcript isoform diversity and safeguards efficient gene expression programs linked to cell-cycle progression and genome maintenance. Perturbation of PHF5A function can drive widespread splicing defects, including aberrant exon usage and intron retention, which reshapes proteostasis and stress-response pathways. Dysregulated spliceosome components, including PHF5A, are frequently studied in the context of cancers and other disorders characterized by altered RNA processing and transcriptional adaptation.
PHF5A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PHF5A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PHF5A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PHF5A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PHF5A protein expression.
This CRISPR knockout system enables efficient generation of PHF5A-deficient cell models for investigation of PHF5A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PHF5A exon(s) critical for PHF5A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PHF5A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PHF5A CRISPR/Cas9 KO Plasmid (h) and PHF5A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PHF5A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PHF5A HDR Plasmid (h) and PHF5A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PHF5A homology arms to support homology-directed repair at defined PHF5A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.