PHC3 CRISPR/Cas9 KO Plasmid (h): sc-406576
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PHC3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PHC3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PHC3 CRISPR/Cas9 KO Plasmid (h) | sc-406576 | 20 µg | $397.00 |
Overview
PHC3 (polyhomeotic homolog 3) is a core component of Polycomb repressive complex 1 (PRC1) that contributes to chromatin compaction and stable transcriptional silencing of developmental and cell identity genes. Through Polycomb-dependent epigenetic regulation, PHC3 participates in maintenance of lineage programs and regulation of proliferation by shaping histone ubiquitination–linked repressive chromatin states. Altered Polycomb activity and dysregulated PRC1-associated repression are frequently implicated in oncogenic transcriptional programs and aberrant differentiation, making PHC3 relevant to studies of tumor biology and epigenetic control. PHC3 function is also used as a readout for understanding how chromatin regulators coordinate gene networks during development and stress responses.
PHC3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PHC3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PHC3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PHC3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PHC3 protein expression.
This CRISPR knockout system enables efficient generation of PHC3-deficient cell models for investigation of PHC3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PHC3 exon(s) critical for PHC3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PHC3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PHC3 CRISPR/Cas9 KO Plasmid (h) and PHC3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PHC3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PHC3 HDR Plasmid (h) and PHC3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PHC3 homology arms to support homology-directed repair at defined PHC3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.