PHC1 Antibody (D-10): sc-390880

PHC1 Antibody (D-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects PHC1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PHC1 (D-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PHC1, also known as polyhomeotic homolog 1, EDR1, HPH1, or RAE28, plays a crucial role in gene expression regulation through involvement in the Polycomb group (PcG) multiprotein complex, specifically the PRC1 complex. This complex maintains transcriptional repression of target genes during development, vital for proper cellular differentiation and function. PHC1 is a 1,004 amino acid nuclear protein that exists as a homodimer and contains a FCS-type zinc finger and a sterile alpha motif (SAM) domain, both important for structural integrity and functional interactions. PHC1 is encoded on human chromosome 12, a region harboring over 1,100 genes and constituting approximately 4.5% of the human genome. PHC1 interacts with several proteins within the PRC1 complex, including Mel-18, Bmi-1, M33, MPc2, MPc3, RING1, and Ring1b, highlighting its integral role in chromatin remodeling and histone modification processes.

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PHC1 Antibody (D-10) References:

1. The core of the polycomb repressive complex is compositionally and functionally conserved in flies and humans.  |  Levine, SS., et al. 2002. Mol Cell Biol. 22: 6070-8. PMID: 12167701
2. Identification and characterisation of novel mammalian homologues of Drosophila polyhomeoticpermits new insights into relationships between members of the polyhomeotic family.  |  Tonkin, E., et al. 2002. Hum Genet. 111: 435-42. PMID: 12384788
3. Gene repression by Polycomb group protein complexes: a distinct complex for every occasion?  |  Otte, AP. and Kwaks, TH. 2003. Curr Opin Genet Dev. 13: 448-54. PMID: 14550408
4. Mammalian polyhomeotic homologues Phc2 and Phc1 act in synergy to mediate polycomb repression of Hox genes.  |  Isono, K., et al. 2005. Mol Cell Biol. 25: 6694-706. PMID: 16024804
5. Differential expression of polycomb repression complex 1 (PRC1) members in the developing mouse brain reveals multiple complexes.  |  Vogel, T., et al. 2006. Dev Dyn. 235: 2574-85. PMID: 16786585
6. PHC3, a component of the hPRC-H complex, associates with E2F6 during G0 and is lost in osteosarcoma tumors.  |  Deshpande, AM., et al. 2007. Oncogene. 26: 1714-22. PMID: 17001316
7. ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.  |  Matsuoka, S., et al. 2007. Science. 316: 1160-6. PMID: 17525332
8. PcG proteins, DNA methylation, and gene repression by chromatin looping.  |  Tiwari, VK., et al. 2008. PLoS Biol. 6: 2911-27. PMID: 19053175
9. Mutation in PHC1 implicates chromatin remodeling in primary microcephaly pathogenesis.  |  Awad, S., et al. 2013. Hum Mol Genet. 22: 2200-13. PMID: 23418308
10. Molecular genetics of human primary microcephaly: an overview.  |  Faheem, M., et al. 2015. BMC Med Genomics. 8 Suppl 1: S4. PMID: 25951892
11. Plastisphere and microorganisms involved in polyurethane biodegradation.  |  Park, WJ., et al. 2023. Sci Total Environ. 886: 163932. PMID: 37156380
12. Acquisition of neural fate by combination of BMP blockade and chromatin modification.  |  Ong, ALC., et al. 2023. iScience. 26: 107887. PMID: 37771660

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PHC1 Antibody (D-10) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-PHC1 Antibody (D-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PHC1 Antibody (D-10) Specifications

Alternate Product Name: PHC1

Product Clone Name: D-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 565-643

Epitope Target: PHC1

Epitope Species: human

Product Reactivity/Detection: PHC1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for PHC1 Antibody (D-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin