PH-4 CRISPR/Cas9 KO Plasmid (h): sc-409966
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PH-4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PH-4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PH-4 CRISPR/Cas9 KO Plasmid (h) | sc-409966 | 20 µg | $397.00 |
Overview
P4HTM encodes prolyl 4-hydroxylase transmembrane protein (PH-4), an endoplasmic reticulum–associated dioxygenase that catalyzes proline hydroxylation on select substrates, linking oxygen and metabolic cofactor availability to post-translational protein modification. As a member of the prolyl 4-hydroxylase family, PH-4 is positioned to influence protein folding, stability, and quality-control processes within the secretory pathway, with downstream effects on cellular stress responses and proteostasis. P4HTM activity has been connected to oxygen-sensing biology and regulation of hypoxia-responsive signaling, integrating with pathways that shape cellular adaptation to reduced oxygen tension. Genetic and functional studies have implicated P4HTM in disease-relevant phenotypes affecting neurodevelopment and metabolic homeostasis, making it a target for mechanistic studies in human cell systems.
PH-4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the P4HTM gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the P4HTM together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the P4HTM open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PH-4 protein expression.
This CRISPR knockout system enables efficient generation of P4HTM-deficient cell models for investigation of PH-4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting P4HTM exon(s) critical for PH-4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple P4HTM genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PH-4 CRISPR/Cas9 KO Plasmid (h) and PH-4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the P4HTM locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PH-4 HDR Plasmid (h) and PH-4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by P4HTM homology arms to support homology-directed repair at defined P4HTM target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.