PGs2 Antibody (D-9): sc-514306

PGs2 Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects PGs2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PGs2 (D-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PGs2, also known as C18orf10, is a 300 amino acid cytoplasmic protein that plays a crucial role in the neuronal tubulin polyglutamylase complex, which is essential for the post-translational modification of tubulin. This modification, particularly polyglutamylation, is vital for several microtubule functions, including axonemal beating, stability of centrioles, and neuronal differentiation, as PGs2 mediates interactions between tubulin and microtubule-associated proteins. The neuronal tubulin polyglutamylase complex, which includes PGs2 along with PGs1, PGs3, PGs4, and PGs5, is characterized by the presence of a TTL (tubulin tyrosine ligase-like) domain that catalyzes the ligation of glutamate to tubulin, thereby enhancing functional capacity. The TTL domain features ATP-grasp-like motifs that correspond to the ATP/Mg(2+) binding site, typical of enzymes with ATP-dependent carboxylate-amine/thiol ligase activity. Anti-PGs2 antibody (D-9) is an invaluable tool for researchers studying the dynamics of microtubule function and the intricate processes of neuronal development and stability.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PGs2 Antibody (D-9) References:

1. Differential binding regulation of microtubule-associated proteins MAP1A, MAP1B, and MAP2 by tubulin polyglutamylation.  |  Bonnet, C., et al. 2001. J Biol Chem. 276: 12839-48. PMID: 11278895
2. Characterisation of PGs1, a subunit of a protein complex co-purifying with tubulin polyglutamylase.  |  Regnard, C., et al. 2003. J Cell Sci. 116: 4181-90. PMID: 12972506
3. Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas.  |  Yamada, S., et al. 2004. Oncogene. 23: 5901-11. PMID: 15221005
4. Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer.  |  Petroziello, J., et al. 2004. Oncogene. 23: 7734-45. PMID: 15334068
5. Tubulin polyglutamylase enzymes are members of the TTL domain protein family.  |  Janke, C., et al. 2005. Science. 308: 1758-62. PMID: 15890843
6. Polyglutamylation: a fine-regulator of protein function? 'Protein Modifications: beyond the usual suspects' review series.  |  Janke, C., et al. 2008. EMBO Rep. 9: 636-41. PMID: 18566597
7. Post-translational modifications of tubulin in the nervous system.  |  Fukushima, N., et al. 2009. J Neurochem. 109: 683-93. PMID: 19250341
8. Polyglutamylation of tubulin as a progressive regulator of in vitro interactions between the microtubule-associated protein Tau and tubulin.  |  Boucher, D., et al. 1994. Biochemistry. 33: 12471-7. PMID: 7522559
9. [Microtubules: functional polymorphisms of tubulin and associated proteins (structural and motor MAP's)].  |  Regnard, C., et al. 1996. C R Seances Soc Biol Fil. 190: 255-68. PMID: 8869236

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PGs2 Antibody (D-9) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PGs2 Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PGs2 Antibody (D-9) Specifications

Alternate Product Name: PGs2

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 48-261

Epitope Target: PGs2

Epitope Species: human

Product Reactivity/Detection: PGs2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PGs2 Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin