Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PGRMC1 Double Nickase Plasmid (h): sc-401945-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PGRMC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PGRMC1 Double Nickase Plasmid (h) and PGRMC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PGRMC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PGRMC1 Antibody (C-4): sc-393015
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PGRMC1 Double Nickase Plasmid (h) | sc-401945-NIC | 20 µg | $410.00 | |||
PGRMC1 Double Nickase Plasmid (h2) | sc-401945-NIC-2 | 20 µg | $410.00 |
Progesterone receptor membrane component 1 (PGRMC1) is a heme-binding membrane-associated protein implicated in nonclassical progesterone signaling, sterol metabolism, and regulation of cytochrome P450 enzyme activity. It localizes predominantly to the endoplasmic reticulum and other membrane compartments, where it influences lipid homeostasis, vesicular trafficking, and cellular stress responses, including pathways linked to proliferation and survival. PGRMC1 has been connected to reproductive biology and metabolic regulation, and altered expression patterns have been reported in multiple disease contexts, including cancer biology and neurodegenerative processes. These features make PGRMC1 a useful target for mechanistic studies of membrane progesterone signaling, redox/heme biology, and P450-centered metabolic networks.
PGRMC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PGRMC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PGRMC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PGRMC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PGRMC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.