PGRMC1 Antibody (C-4) recommended for detection of PGRMC1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

PGRMC1 Antibody (C-4): sc-393015

PGRMC1 Antibody (C-4) is rated 5.0 out of 5 by 12.
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Datasheets

    • Anti-PGRMC1 Antibody (C-4) is a mouse monoclonal IgM (kappa light chain) PGRMC1 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 61-92 within an internal region of PGRMC1 of human origin
    • recommended for detection of PGRMC1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • TransCruz reagent for ChIP application (sc-393015 X, 200 µg/0.1 ml)
    • blocking peptide, sc-393015 P
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PGRMC1 (C-4): sc-393015.
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Hi. We have stained kidney samples with PGRMC1 antibody (sc-393015, Lot #2217) but the results is different from the brochure (gromerulus: negative, tubules: positive). Is the lot wrong? How can I go ahead with the staining?

Asked by: Knishi
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-06-16

What application is the blocking peptide sc-393015 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent validated antibody The SCBT PGRMC1 antibody (Sc393015) detects the native PGRMC1 protein at ~22 kDa and several post translational modified forms at ~65 and ~110 kDa molecular weight equivalent bands in HEK293 total cell extracts (see the attached immunoblot picture). The higher molecular weight bands are possibly sumoylated forms (see Peluso et al. 2012, PMID:22719051) and trafficked to different cell organelles. The antibody is also equally good in immunofluorescence study (see the attached image). The antibody detected ER and nuclear localized PGRMC1 which has been previously reported by Human Protein Atlas PGRMC1 validated Sigma antibody HPA002877. Interestingly this SCBT antibody is comparable (Immunoblot and immunofluorescence) to the Protein atlas validated antibody Sigma, but the price is half of the Sigma antibody. So, I would highly recommend this as a cost effective and efficient antibody for your research. Go ahead and use this antibody for your research and make great discoveries. It’s an important protein.
Date published: 2018-03-02
Rated 5 out of 5 by from Western blot analysis Western blot analysis PGRMC1 ,loading 40ug rat kidney lysates,positve band obtained at 27kda.
Date published: 2017-09-03
Rated 5 out of 5 by from clear band We use 1:500 dilution , the band is very clear and specific. Very satisfied! The antibody works in accordance with expectation.
Date published: 2017-08-18
Rated 5 out of 5 by from western blot detection This antibody was used for the western blot detection of mouse PGRMC1 in primary lymphocytes. These cells were grown in RPMI with 10% FBS. This antibody is very specific and no background was seen in whole cell lysates.
Date published: 2017-07-29
Rated 5 out of 5 by from The antibody is very nice This antibody been used for analysis of PGRMC expressin in human liver cell line,it gave the strong target band at 28kda when use the condition 1:400 for incubated 1hour at room temperature..Since it is IgM antibody.I recommend use the anti mouse IgM secondary.
Date published: 2017-07-08
Rated 5 out of 5 by from nice staining IHC of paraffin-embedded human tissue showed nice staining with no background,dilution 1:300 shows strong signal.
Date published: 2017-05-13
Rated 5 out of 5 by from Immunofluorescence Nice immunofluorescence staining in human adrenal cells.
Date published: 2017-02-14
Rated 5 out of 5 by from very great This PGRMC1 antibody is very great with high quanlity and high specificity for wb,at1:500
Date published: 2017-02-12
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