Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Peroxin 3 Double Nickase Plasmid (h): sc-405639-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Peroxin 3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Peroxin 3 Double Nickase Plasmid (h) and Peroxin 3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PEX3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Peroxin 3 Antibody (B-6): sc-515149
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Peroxin 3 Double Nickase Plasmid (h) | sc-405639-NIC | 20 µg | $410.00 |
PEX3 encodes peroxin 3, an essential membrane protein that initiates peroxisome biogenesis by recruiting cytosolic peroxins and enabling insertion of peroxisomal membrane proteins into the developing organelle. Through its role in peroxisomal assembly, PEX3 supports lipid metabolic pathways including very-long-chain fatty acid β-oxidation, plasmalogen biosynthesis, and reactive oxygen species homeostasis. Disruption of PEX3 compromises peroxisome formation and perturbs cellular redox balance and membrane lipid composition, processes that are central to metabolic adaptation in many cell types. Pathogenic loss-of-function variants in PEX3 are linked to peroxisome biogenesis disorders within the Zellweger spectrum, underscoring its relevance to studies of inherited metabolic disease mechanisms.
Peroxin 3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PEX3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PEX3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PEX3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PEX3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.