Peroxin 3 CRISPR/Cas9 KO Plasmid (m): sc-425227
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Peroxin 3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Peroxin 3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Peroxin 3 CRISPR/Cas9 KO Plasmid (m) | sc-425227 | 20 µg | $397.00 |
Overview
Pex3 encodes peroxin 3, a core peroxisome biogenesis factor that localizes to the peroxisomal membrane and helps initiate membrane assembly by recruiting additional peroxins required for matrix protein import. Through its role in peroxisome formation, PEX3 supports lipid metabolic pathways including very-long-chain fatty acid β-oxidation, ether phospholipid (plasmalogen) synthesis, and reactive oxygen species detoxification. Loss of peroxisomal function disrupts cellular redox homeostasis and membrane lipid composition, impacting mitochondrial crosstalk and inflammatory signaling. In mice, Pex3 is frequently studied to model peroxisome biogenesis defects and to probe how peroxisome loss influences neurodevelopment, hepatocellular metabolism, and systemic energy balance.
Peroxin 3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pex3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pex3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pex3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Peroxin 3 protein expression.
This CRISPR knockout system enables efficient generation of Pex3-deficient cell models for investigation of Peroxin 3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pex3 exon(s) critical for Peroxin 3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pex3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Peroxin 3 CRISPR/Cas9 KO Plasmid (m) and Peroxin 3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pex3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Peroxin 3 HDR Plasmid (m) and Peroxin 3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pex3 homology arms to support homology-directed repair at defined Pex3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.