
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Periostin Lentiviral Activation Particles (m) | sc-424463-LAC | 200 µl | $455.00 |
Postn encodes periostin, a secreted extracellular matrix matricellular protein that binds integrins and modulates cell adhesion, migration, and survival during tissue remodeling. In mouse, periostin contributes to collagen fibrillogenesis and extracellular matrix organization, integrating mechanical cues with signaling pathways such as FAK/PI3K–AKT, TGF-β/SMAD, and Wnt-associated programs. It is highly responsive to injury and inflammatory stimuli and is frequently studied in fibrosis, osteogenesis, angiogenesis, and tumor–stroma interactions. Altered Postn expression and periostin deposition are linked to changes in fibroblast activation and epithelial–mesenchymal crosstalk relevant to chronic remodeling phenotypes.
Periostin Lentiviral Activation Particles (m) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient Postn upregulation across a broader range of human cell types.
Periostin Lentiviral Activation Particles (m) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the Postn transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous Periostin expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native Postn genomic locus and regulatory architecture.
The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.