Perforin 1 Antibody (E-5): sc-374346

Perforin 1 Antibody (E-5) is a mouse monoclonal IgG2b antibody that detects perforin 1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Perforin 1 plays a crucial role in the immune response by facilitating the destruction of virus-infected and tumorigenic cells through cytotoxic T lymphocyte (CTL)-mediated cytotoxicity. Perforin 1 is primarily located in cytotoxic T lymphocytes and natural killer cells, where secretion occurs in granules during immune responses. Perforin 1 forms pores in the membranes of target cells, allowing the entry of serine proteases known as granzymes, which induce apoptosis. Perforin 1 has been identified in astrocytes within the human brain, suggesting a potential role in neuroimmune interactions. Various cytokines influence perforin 1 activity, including interleukin-2, interleukin-3, interleukin-4, and interleukin-6, highlighting its importance in modulating immune responses. Understanding perforin 1 function and localization is essential for elucidating its role in both protective immunity and pathological conditions such as autoimmune diseases and transplant rejection.

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Perforin 1 Antibody (E-5) References:

1. Small changes in the regulation of one Arabidopsis profilin isovariant, PRF1, alter seedling development.  |  McKinney, EC., et al. 2001. Plant Cell. 13: 1179-91. PMID: 11340190
2. Six novel mutations in the PRF1 gene in children with haemophagocytic lymphohistiocytosis.  |  Clementi, R., et al. 2001. J Med Genet. 38: 643-6. PMID: 11565555
3. Human microRNA-27a* targets Prf1 and GzmB expression to regulate NK-cell cytotoxicity.  |  Kim, TD., et al. 2011. Blood. 118: 5476-86. PMID: 21960590
4. Properties of a purified pore-forming protein (perforin 1) isolated from H-2-restricted cytotoxic T cell granules.  |  Young, JD., et al. 1986. J Exp Med. 164: 144-55. PMID: 2425027
5. A novel PRF1 gene mutation in a fatal neonate case with type 2 familial hemophagocytic lymphohistiocytosis.  |  Kim, JY., et al. 2014. Korean J Pediatr. 57: 50-3. PMID: 24578718
6. Accuracy of flow cytometric perforin screening for detecting patients with FHL due to PRF1 mutations.  |  Abdalgani, M., et al. 2015. Blood. 126: 1858-60. PMID: 26450956
7. Reduced PRF1 enhancer methylation in children with a history of severe RSV bronchiolitis in infancy: an association study.  |  Elgizouli, M., et al. 2017. BMC Pediatr. 17: 65. PMID: 28253869
8. Familial Hemophagocytic Lymphohistiocytosis Secondary to PRF1 Mutation.  |  Alfaraidi, AT., et al. 2021. Case Rep Hematol. 2021: 7213939. PMID: 35003815
9. First case of very late-onset FHL2 in Spain with two variants in the PRF1 gene.  |  Sienes Bailo, P., et al. 2023. Ann Clin Biochem. 60: 356-364. PMID: 37365821
10. PRF1 as a prognostic gene for lymphatic metastasis in skin melanoma.  |  Lin, Y., et al. 2024. Biochem Biophys Res Commun. 734: 150338. PMID: 39083978

The preferred Perforin 1 antibody is Perforin 1 Antibody (F-1): sc-136994.