Perforin 1 Antibody (δG9): sc-33655
- Perforin 1 Antibody (δG9) is a mouse monoclonal IgG2b κ, cited in 3 publications, provided at 200 µg/ml
- raised against purified granules from the human lymphoma line YT
- recommended for detection of Perforin 1 of human and rat origin by WB, IP, IF and FCM; non cross-reactive with mouse Perforin 1
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Perforin 1 Antibody (δG9) for WB applications. These reagents are now offered in bundles with Perforin 1 Antibody (δG9) (see ordering information below).
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Perforin 1 Antibody (δG9) is a mouse monoclonal IgG2b antibody that detects perforin 1 in human and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Perforin 1 plays a crucial role in the immune response by facilitating the destruction of virus-infected and tumorigenic cells through cytotoxic T lymphocyte (CTL)-mediated cytotoxicity. Perforin 1 is primarily located in cytotoxic T lymphocytes and natural killer cells, where perforin 1 is secreted in response to immune activation. Perforin 1 in these immune cells is vital, as perforin 1 forms pores in the membranes of target cells, allowing the entry of cytotoxic serine proteases like granzymes, which induce apoptosis. Perforin 1 has also been identified in astrocytes within the human brain, suggesting a potential role in neuroimmune interactions. Perforin 1 activity is regulated by various cytokines, including interleukins 2, 3, 4, and 6, as well as tumor necrosis factor and interferon-gamma, highlighting perforin 1′s importance in both immune defense and the modulation of inflammatory responses. Anti-perforin 1 antibody (δG9) is an essential tool for researchers studying immune responses, cell-mediated cytotoxicity, and the underlying mechanisms of autoimmune diseases and transplant rejection.
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Perforin 1 Antibody (δG9) References:
- Ectopically expressed perforin-1 is proapoptotic in tumor cell lines by increasing caspase-3 activity and the nuclear translocation of cytochrome C. | Wang, LF., et al. 2012. PLoS One. 7: e40639. PMID: 22829880
- Isolation and biochemical and functional characterization of perforin 1 from cytolytic T-cell granules. | Podack, ER., et al. 1985. Proc Natl Acad Sci U S A. 82: 8629-33. PMID: 2417226
- [Association between gene polymorphisms of Perforin 1 and hemophagocytic lymphohistiocytosis]. | Huang, XH., et al. 2015. Zhongguo Dang Dai Er Ke Za Zhi. 17: 677-82. PMID: 26182270
- Perforin gene PRF1 c.900C> T polymorphism and HIV-1 vertical transmission. | Zupin, L., et al. 2019. Genet Mol Biol. 42: 574-577. PMID: 31188937
- Structure, function and expression of murine and human perforin 1 (P1). | Podack, ER., et al. 1988. Immunol Rev. 103: 203-11. PMID: 3292394
- Frequent birth-and-death events throughout perforin-1 evolution. | Araujo-Voces, M. and Quesada, V. 2020. BMC Evol Biol. 20: 135. PMID: 33076840
- Breaching the Bacterial Envelope: The Pivotal Role of Perforin-2 (MPEG1) Within Phagocytes. | Merselis, LC., et al. 2021. Front Immunol. 12: 597951. PMID: 33692780
- Identification and functional analysis of the perforin-1 like gene in disease resistance in half smooth tongue sole (Cynoglossus semilaevis). | Fu, X., et al. 2021. Dev Comp Immunol. 122: 104135. PMID: 34004267
- Identification and functional analysis of perforin 1 from largemouth bass (Micropterus salmoides). | Hu, X., et al. 2024. Fish Shellfish Immunol. 149: 109531. PMID: 38604479
- Perforin 1 in Cancer: Mechanisms, Therapy, and Outlook. | Guan, X., et al. 2024. Biomolecules. 14: PMID: 39199299
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Perforin 1 Antibody (δG9) | sc-33655 | 200 µg/ml | $322.00 | |||
Perforin 1 Antibody (δG9): m-IgG Fc BP-HRP Bundle | sc-536770 | 200 µg Ab; 10 µg BP | $361.00 | |||
Perforin 1 Antibody (δG9): m-IgGκ BP-HRP Bundle | sc-533934 | 200 µg Ab; 40 µg BP | $361.00 | |||
Perforin 1 Antibody (δG9): m-IgG2b BP-HRP Bundle | sc-549826 | 200 µg Ab; 10 µg BP | $361.00 | |||
Perforin 1 Antibody (δG9) FITC | sc-33655 FITC | 200 µg/ml | $336.00 | |||
Perforin 1 Antibody (δG9) PE | sc-33655 PE | 200 µg/ml | $349.00 |