Percoll® Low viscosity density gradient medium for preparation of cells, subcellular particles, and larger viruses.


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Alternate Names: Percoll® also known as Percoll® Density Gradient Media
Application: Percoll® is low viscosity density gradient medium for preparation of cells, subcellular particles, and larger viruses
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

Percoll® is used in density centrifugation applications for the separation of cells, subcellular particles and larger viruses (down to ~ 70S) under gentle conditions which preserve viability and morphological integrity. Percoll® consists of silica particles (15 to 30 nm diameter) coated with non-dialyzable polyvinylpyrrolidone (PVP). Free PVP is present at only 1% to 2%. Percoll® is non-toxic, almost chemically inert and does not adhere to membranes. Percoll® is adjustable to physiological ionic strength and pH, and gradients can either be performed or spontaneously generated by centrifugation at moderate speeds in an angle-head rotor. Gradients are iso-osmotic throughout and cover a range of densities up to 1.3 g/ml.

Percoll® has also been used in sperm preparation to study the energy requirements of bovine embryos.

Usage :
If stored at -20°C, gradients form upon thawing, necessitating a mixing of the bottle before use. Performed gradients can be stored for weeks without a change in gradient shape, provided that the gradient remains unfrozen.

⋅ Percoll® can be buffered within the pH range 5.5 to 10.0 without any changes in properties. If the pH is dropped below 5.5, gelling may occur. Gelling can also be caused by the presence of divalent cations, an effect which is exacerbated by elevated temperatures.

⋅ Undiluted Percoll® can be resterilized by autoclaving for 30 min at 120°C.

Preparation of gradient material

Percoll® PLUS (sc-296039)/Percoll® are best used in balanced salt solutions, physiological saline, or 0.25 M sucrose. Cells can be separated on gradients in balanced salt solutions. Subcellular particles, however, tend to aggregate in the presence of salts and it is recommended that the separation of such particles be carried out in Percoll®diluted with sucrose (0.25 M final concentration).

The low osmolality of Percoll® permits this parameter to be controlled by the user without significant interference from the density medium itself. The addition of 9 parts (v/v) of Percoll® to 1 part (v/v) of either 1.5 M NaCl, 10x concentrated cell culture medium, or 2.5 M sucrose will result in a solution adjusted to about 340 mOsm/kg H2O. Solutions of different osmotic pressure can be produced by adjusting the relative volumes of Percoll® and salt or sucrose solution. The final adjustment to the required osmolality can be carried out by the addition of salts or distilled water. When precise osmotic pressures are required, it is recommended that the osmolality of the solutions be measured in an osmometer.

Centrifugation with Percoll®

Percoll® will form self-generated gradients by
centrifugation at approximately 10,000 gav (in 0.15 M saline) or 25,000 gav (in 0.25 M sucrose) in fixed-angle rotor heads after 15 minutes. Cells or subcellular particles can be mixed with Percoll® prior to centrifugation and will band isopycnically, as the gradient is formed in situ. Although Percoll® is best used in angle-head rotors, banding of cells on pre-formed (continuous or discontinuous) gradients may be carried out at 400 gav for 20 to 30 minutes in swing-out rotors.

Removal of Percoll®after centrifugation

To remove the gradient medium from the biological material, perform one of the procedures outlined below.
• Cells can be recovered free from particles of Percoll® by dilution with physiological saline and centrifugation to collect the cells.
• Subcellular particles can be separated from Percoll® by the procedure described above. The size of the particles will determine the centrifugal force required to separate the particles from Percoll®.
• Gel filtration or ion exchange chromatography can also be used to separate biological material from Percoll®.

Aggregates of silica particles

It is an inherent tendency of all silica colloids to form aggregates on prolonged storage. These aggregates may be observed in some batches of Percoll®, either as a slight sediment at the bottom of the tube or as a faint white band with a density of 1.04 to 1.05 g/ml. This band may form during gradient formation in the centrifuge or during low speed centrifugation of a performed gradient. The aggregated silica does not interfere with the separation of biological particles and almost all cells and organelles have buoyant densities of greater than 1.05 g/ml in PPercoll® PLUS For the majority of cell, virus, and organelle separations, any silica aggregates banded from the gradient material (see above) may be ignored.
For specific experiments, it may be desirable to remove aggregates; this may be achieved by filtration of Percoll® through a depth filter prior to centrifugation.
Aggregation is not a problem in Percoll® PLUS (sc-296039) due to the silane coating.
Formulation :
Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)
Appearance :
Physical State :
pH :
8.5 - 9.5
Storage :
Store at room temperature
Density :
max: 1.135 g/mL
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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Percoll®  Product Citations

See how others have used Percoll®. Click on the entry to view the PubMed entry .

Citations 1 to 4 of 4 total

PMID: # 33546594  Fatemi, A.|Alipour, R.|Khanahmad, H.|Alsahebfosul, F.|Andalib, A.|Pourazar, A.| et al. 2021. BMC Immunol. 22: 12.

PMID: # 26113472  Shintani-Ishida, K. et al. 2015. Int. J. Cardiol. 197: 26-32.

PMID: # 33111119  STAR Protoc. 100086.

PMID: # 31452708  Exp Ther Med. 18: 2167-2177.

Citations 1 to 4 of 4 total

Hur många liter Percoll säljer ni per år? 

Asked by: Karl
Thank you for your question. Unfortunately this is proprietary information. Please contact us for any further information!
Answered by: Tech Service
Date published: 2018-09-07
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