Pepsin A4 CRISPR/Cas9 KO Plasmid (h): sc-418415
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pepsin A4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Pepsin A4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pepsin A4 CRISPR/Cas9 KO Plasmid (h) | sc-418415 | 20 µg | $397.00 |
Overview
PGA4 encodes pepsin A4, an aspartic endopeptidase produced as a secreted zymogen in gastric mucosa and activated under acidic conditions to cleave dietary and endogenous proteins. As a member of the pepsin family, pepsin A4 contributes to gastrointestinal proteostasis, influencing peptide generation, luminal protein digestion, and downstream antigen and nutrient availability. Its activity is integrated with gastric acid biology and mucosal barrier processes, and altered expression or protease balance is often evaluated in studies of gastric epithelial remodeling and inflammation-associated pathology. PGA4 is therefore relevant for dissecting protease-dependent pathways in the stomach and for biomarker-oriented research in gastric disease contexts.
Pepsin A4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PGA4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PGA4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PGA4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Pepsin A4 protein expression.
This CRISPR knockout system enables efficient generation of PGA4-deficient cell models for investigation of Pepsin A4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PGA4 exon(s) critical for Pepsin A4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PGA4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Pepsin A4 CRISPR/Cas9 KO Plasmid (h) and Pepsin A4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PGA4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Pepsin A4 HDR Plasmid (h) and Pepsin A4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PGA4 homology arms to support homology-directed repair at defined PGA4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.