PELO Double Nickase Plasmid (h): sc-405192-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PELO Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PELO Double Nickase Plasmid (h) and PELO Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PELO. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PELO Antibody (F-4): sc-393418
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PELO Double Nickase Plasmid (h) | sc-405192-NIC | 20 µg | $410.00 | |||
PELO Double Nickase Plasmid (h2) | sc-405192-NIC-2 | 20 µg | $410.00 |
PELO (pelota homolog) encodes a conserved ribosome rescue factor that recognizes stalled translating ribosomes and promotes resolution of aberrant mRNAs, supporting global translation homeostasis and mRNA surveillance. PELO functions with HBS1L in the no-go decay and ribosome quality control pathways, facilitating dissociation of stalled 80S complexes and recycling of ribosomal subunits. Through its role in proteostasis and stress adaptation, altered PELO activity can influence cell-cycle control, differentiation programs, and susceptibility to proteotoxic and genotoxic stress. Dysregulation of translation quality control networks that involve PELO has been associated with neurodevelopmental phenotypes and cancer-related cellular states, making it relevant for mechanistic studies of RNA metabolism and protein synthesis fidelity.
PELO Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PELO locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PELO. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PELO function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PELO-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.