PELO Antibody (F-4): sc-393418
- PELO Antibody (F-4) is a mouse monoclonal IgG2a κ PELO antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-231 mapping at the N-terminus of PELO of human origin
- PELO Antibody (F-4) is recommended for detection of PELO of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PELO Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PELO Antibody (F-4) for WB applications. These reagents are now offered in bundles with PELO Antibody (F-4) (see ordering information below).
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PELO Antibody (F-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects PELO protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PELO (F-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PELO protein, also known as pelota homolog, CGI-17, or PRO1770, is a 385 amino acid protein located in both the nucleus and cytoplasm, belonging to the eukaryotic release factor 1 family and the pelota subfamily. Evolutionary conservation highlights PELO′s critical role in cellular processes, particularly in the regulation of cell proliferation and stem cell self-renewal. PELO is essential for normal chromosome segregation during cell division, which is vital for maintaining genomic stability. PELO may exhibit ribonuclease activity, enabling recognition of stalled ribosomes and triggering endonucleolytic cleavage of mRNA, a mechanism that facilitates the degradation of damaged mRNAs and release of non-functional ribosomes. This function underscores PELO′s importance in cellular quality control and overall integrity of the translational machinery. PELO is ubiquitously expressed and requires divalent metal cations as cofactors, indicating involvement in various cellular contexts, including spermatogenesis and meiotic cell division. The gene encoding PELO is located on human chromosome 5q11.2, further emphasizing significance in human biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PELO Antibody (F-4) References:
- Molecular cloning, expression and chromosome location of the human pelota gene PELO. | Shamsadin, R., et al. 2000. Cytogenet Cell Genet. 90: 75-8. PMID: 11060452
- Mouse pelota gene (Pelo): cDNA cloning, genomic structure, and chromosomal localization. | Shamsadin, R., et al. 2002. Cytogenet Genome Res. 97: 95-9. PMID: 12438745
- Disruption of the pelota gene causes early embryonic lethality and defects in cell cycle progression. | Adham, IM., et al. 2003. Mol Cell Biol. 23: 1470-6. PMID: 12556505
- Pelota controls self-renewal of germline stem cells by repressing a Bam-independent differentiation pathway. | Xi, R., et al. 2005. Development. 132: 5365-74. PMID: 16280348
- Pelota interacts with HAX1, EIF3G and SRPX and the resulting protein complexes are associated with the actin cytoskeleton. | Burnicka-Turek, O., et al. 2010. BMC Cell Biol. 11: 28. PMID: 20406461
- The pelota locus encodes a protein required for meiotic cell division: an analysis of G2/M arrest in Drosophila spermatogenesis. | Eberhart, CG. and Wasserman, SA. 1995. Development. 121: 3477-86. PMID: 7588080
- An archaebacterial homolog of pelota, a meiotic cell division protein in eukaryotes. | Ragan, MA., et al. 1996. FEMS Microbiol Lett. 144: 151-5. PMID: 8900058
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PELO Antibody (F-4) | sc-393418 | 200 µg/ml | $322.00 | |||
PELO Antibody (F-4): m-IgGκ BP-HRP Bundle | sc-523937 | 200 µg Ab, 40 µg BP | $361.00 | |||
PELO Antibody (F-4): m-IgG2a BP-HRP Bundle | sc-546171 | 200 µg Ab; 10 µg BP | $361.00 | |||
PELO Antibody (F-4) AC | sc-393418 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PELO Antibody (F-4) HRP | sc-393418 HRP | 200 µg/ml | $322.00 | |||
PELO Antibody (F-4) FITC | sc-393418 FITC | 200 µg/ml | $336.00 | |||
PELO Antibody (F-4) PE | sc-393418 PE | 200 µg/ml | $349.00 | |||
PELO Antibody (F-4) Alexa Fluor® 488 | sc-393418 AF488 | 200 µg/ml | $364.00 | |||
PELO Antibody (F-4) Alexa Fluor® 546 | sc-393418 AF546 | 200 µg/ml | $364.00 | |||
PELO Antibody (F-4) Alexa Fluor® 594 | sc-393418 AF594 | 200 µg/ml | $364.00 | |||
PELO Antibody (F-4) Alexa Fluor® 647 | sc-393418 AF647 | 200 µg/ml | $364.00 | |||
PELO Antibody (F-4) Alexa Fluor® 680 | sc-393418 AF680 | 200 µg/ml | $364.00 | |||
PELO Antibody (F-4) Alexa Fluor® 790 | sc-393418 AF790 | 200 µg/ml | $364.00 |