PDPK1 Antibody (D-5): sc-376586

PDPK1 Antibody (D-5) is a mouse monoclonal IgG1 antibody that detects PDPK1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PDPK1, also known as 3-phosphoinositide dependent protein kinase 1, is a 556 amino acid protein that is ubiquitously expressed and localizes to the cell membrane, cytoplasm, and nucleus, playing a crucial role in various cellular processes. As a master kinase, PDPK1 is essential for phosphorylating and activating a subgroup of the AGC family of protein kinases, which are pivotal in mediating signal transduction pathways that control cell proliferation, survival, and growth, particularly in developing pancreatic beta cells. Additionally, PDPK1 regulates calcium uptake and calcium-activated potassium channels in mast cells, influences chemotaxis and motility in vascular endothelial cells, and is vital for cardiac homeostasis and thymocyte development. PDPK1′s structure includes a PH domain that is critical for homodimerization, localization, and nuclear import, alongside a protein kinase domain that facilitates enzymatic activity. PDPK1 exists as five alternatively spliced isoforms and is encoded by a gene located on human chromosome 16p13.3, highlighting its complexity and importance in cellular signaling networks.

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PDPK1 Antibody (D-5) References:

1. The 16p13.3 (PDPK1) Genomic Gain in Prostate Cancer: A Potential Role in Disease Progression.  |  Choucair, KA., et al. 2012. Transl Oncol. 5: 453-60. PMID: 23401739
2. Epigenetic repression of miR-375 is the dominant mechanism for constitutive activation of the PDPK1/RPS6KA3 signalling axis in multiple myeloma.  |  Tatekawa, S., et al. 2017. Br J Haematol. 178: 534-546. PMID: 28439875
3. Chlamydia trachomatis Prevents Apoptosis Via Activation of PDPK1-MYC and Enhanced Mitochondrial Binding of Hexokinase II.  |  Al-Zeer, MA., et al. 2017. EBioMedicine. 23: 100-110. PMID: 28803120
4. The enhancement of combination of berberine and metformin in inhibition of DNMT1 gene expression through interplay of SP1 and PDPK1.  |  Zheng, F., et al. 2018. J Cell Mol Med. 22: 600-612. PMID: 28840963
5. miR-718 is involved in malignancy of papillary thyroid cancer through repression of PDPK1.  |  Wang, X. and Qi, M. 2018. Pathol Res Pract. 214: 1787-1793. PMID: 30166214
6. miR‑16‑2‑3p inhibits cell proliferation and migration and induces apoptosis by targeting PDPK1 in maxillary primordium mesenchymal cells.  |  Han, T., et al. 2019. Int J Mol Med. 43: 1441-1451. PMID: 30664182
7. LncRNA KTN1-AS1 promotes the progression of non-small cell lung cancer via sponging of miR-130a-5p and activation of PDPK1.  |  Li, C., et al. 2020. Oncogene. 39: 6157-6171. PMID: 32820252
8. Extracellular vesicle-derived circCEBPZOS attenuates postmyocardial infarction remodeling by promoting angiogenesis via the miR-1178-3p/PDPK1 axis.  |  Yu, L., et al. 2023. Commun Biol. 6: 133. PMID: 36726025
9. Chicoric Acid Presented NLRP3-Mediated Pyroptosis through Mitochondrial Damage by PDPK1 Ubiquitination in an Acute Lung Injury Model.  |  Zhang, W., et al. 2023. Am J Chin Med. 51: 1431-1457. PMID: 37530505
10. In Vitro and Vivo Experiments Revealing Astragalin Inhibited Lung Adenocarcinoma Development via LINC00582/miR-140-3P/PDPK1.  |  Bai, J., et al. 2024. J Biochem Mol Toxicol. 38: e70042. PMID: 39552470

The preferred PDPK1 antibody is PDPK1 Antibody (E-3): sc-17765.