Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PDK1 Antibody (E-10): sc-515944. Western blot analysis of PDK1 expression in non-transfected 293T: sc-117752 (A) and human PDK1 transfected 293T: sc-113873 (B) whole cell lysates and rat heart tissue extract (C).
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PDK1 CRISPR/Cas9 KO Plasmid (h2): sc-401084-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PDK1 CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PDK1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PDK1 Antibody (E-10): sc-515944
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDK1 CRISPR/Cas9 KO Plasmid (h2) | sc-401084-KO-2 | 20 µg | $397.00 |