PDK1 Antibody (E-10): sc-515944

PDK1 Antibody (E-10) is a mouse monoclonal IgM antibody that detects PDK1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PDK1 antibody (E-10) is offered in a non-conjugated form, allowing for versatile experimental setups. PDK1, or pyruvate dehydrogenase kinase 1, plays a crucial role in cellular metabolism by regulating the activity of the mitochondrial pyruvate dehydrogenase complex, which is essential for converting pyruvate into acetyl-CoA, a key substrate for the citric acid cycle. This regulation is vital for maintaining energy homeostasis, particularly in response to varying nutrient availability. The activity of PDK1 is influenced by several factors, including the levels of ATP and NADH, which signal the energy status of the cell. PDK1 undergoes post-translational modifications, such as phosphorylation, which can modulate activity and stability, thereby impacting metabolic pathways. Understanding the function and regulation of PDK1 is essential for elucidating its role in metabolic diseases and potential therapeutic targets.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PDK1 Antibody (E-10) References:

1. Purification and comparative study of the kinases specific for branched chain alpha-ketoacid dehydrogenase and pyruvate dehydrogenase.  |  Popov, KM., et al. 1991. Protein Expr Purif. 2: 278-86. PMID: 1821799
2. A unifying mechanism for stimulation of mammalian pyruvate dehydrogenase(a) kinase by reduced nicotinamide adenine dinucleotide, dihydrolipoamide, acetyl coenzyme A, or pyruvate.  |  Cate, RL. and Roche, TE. 1978. J Biol Chem. 253: 496-503. PMID: 201637
3. Alpha-keto acid dehydrogenase complexes. X. Regulation of the activity of the pyruvate dehydrogenase complex from beef kidney mitochondria by phosphorylation and dephosphorylation.  |  Linn, TC., et al. 1969. Proc Natl Acad Sci U S A. 62: 234-41. PMID: 4306045
4. -Keto acid dehydrogenase complexes. XVII. Kinetic and regulatory properties of pyruvate dehydrogenase kinase and pyruvate dehydrogenase phosphatase from bovine kidney and heart.  |  Hucho, F., et al. 1972. Arch Biochem Biophys. 151: 328-40. PMID: 4339797
5. Purification and properties of pyruvate dehydrogenase kinase from bovine kidney.  |  Stepp, LR., et al. 1983. J Biol Chem. 258: 9454-8. PMID: 6348044
6. Diversity of the pyruvate dehydrogenase kinase gene family in humans.  |  Gudi, R., et al. 1995. J Biol Chem. 270: 28989-94. PMID: 7499431
7. Regulation of the pyruvate dehydrogenase multienzyme complex.  |  Behal, RH., et al. 1993. Annu Rev Nutr. 13: 497-520. PMID: 8369156

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PDK1 Antibody (E-10) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-PDK1 Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PDK1 Antibody (E-10) Specifications

Alternate Product Name: PDK1

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 410-436

Epitope Target: PDK1

Epitope Species: human

Product Reactivity/Detection: precursor and mature PDK1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for PDK1 Antibody (E-10): N/A