Pdcd-4 Antibody (B-4): sc-376430

Pdcd-4 Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Pdcd-4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Pdcd-4 antibody (B-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pdcd-4, also known as Programmed Cell Death 4, plays a crucial role in regulating apoptosis and functions as a tumor suppressor gene, particularly in lung cancer. Pdcd-4 inhibits the transformation of mouse keratinocytes induced by tumor promoters, highlighting its importance in cancer biology. Pdcd-4 is primarily located in the nucleus, where Pdcd-4 interacts with key regulatory proteins involved in gene expression and translation. Notably, Pdcd-4 directly binds to RNA helicase eIF4A, inhibiting protein synthesis by disrupting the formation of the cap-dependent translation initiation complex. This interaction underscores Pdcd-4′s significance in controlling cellular growth and proliferation, as Pdcd-4 also suppresses c-Jun activity, a critical transcription factor involved in oncogenic signaling pathways. By blocking c-Jun′s recruitment of co-activator p300 and affecting phosphorylation, Pdcd-4 (B-4) antibody serves as a valuable tool for studying tumor suppression mechanisms and regulatory networks governing cell fate decisions.

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Pdcd-4 Antibody (B-4) References:

1. The transformation suppressor protein Pdcd4 shuttles between nucleus and cytoplasm and binds RNA.  |  Böhm, M., et al. 2003. Oncogene. 22: 4905-10. PMID: 12894233
2. Programmed cell death protein 4 (pdcd4): a novel target for antineoplastic therapy?  |  Lankat-Buttgereit, B. and Göke, R. 2003. Biol Cell. 95: 515-9. PMID: 14630388
3. Pdcd4 inhibits growth of tumor cells by suppression of carbonic anhydrase type II.  |  Lankat-Buttgereit, B., et al. 2004. Mol Cell Endocrinol. 214: 149-53. PMID: 15062553
4. Programmed cell death protein 4 (PDCD4) acts as a tumor suppressor in neuroendocrine tumor cells.  |  Göke, R., et al. 2004. Ann N Y Acad Sci. 1014: 220-1. PMID: 15153438
5. Transformation suppressor protein Pdcd4 interferes with JNK-mediated phosphorylation of c-Jun and recruitment of the coactivator p300 by c-Jun.  |  Bitomsky, N., et al. 2004. Oncogene. 23: 7484-93. PMID: 15334056
6. Induction of PDCD4 tumor suppressor gene expression by RAR agonists, antiestrogen and HER-2/neu antagonist in breast cancer cells. Evidence for a role in apoptosis.  |  Afonja, O., et al. 2004. Oncogene. 23: 8135-45. PMID: 15361828
7. Pdcd4, a colon cancer prognostic that is regulated by a microRNA.  |  Allgayer, H. 2010. Crit Rev Oncol Hematol. 73: 185-91. PMID: 19836969
8. The role of Pdcd4 in tumour suppression and protein translation.  |  Wang, Q. and Yang, HS. 2018. Biol Cell.. PMID: 29806708
9. The mechanisms and clinical significance of PDCD4 in colorectal cancer.  |  Long, J., et al. 2019. Gene. 680: 59-64. PMID: 30243936
10. Control Mechanisms of the Tumor Suppressor PDCD4: Expression and Functions.  |  Matsuhashi, S., et al. 2019. Int J Mol Sci. 20: PMID: 31075975
11. Human tumor suppressor protein Pdcd4 binds at the mRNA entry channel in the 40S small ribosomal subunit.  |  Brito Querido, J., et al. 2024. Nat Commun. 15: 6633. PMID: 39117603
12. PDCD4 promotes inflammation/fibrosis by activating the PPAR‑γ/NF‑κB pathway in mouse atrial myocytes.  |  Yu, L., et al. 2024. Mol Med Rep. 30: PMID: 39301631

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pdcd-4 Antibody (B-4) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-Pdcd-4 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pdcd-4 Antibody (B-4) Specifications

Alternate Product Name: PDCD4

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 147-317

Epitope Target: Pdcd-4

Epitope Species: human

Product Reactivity/Detection: Pdcd-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for Pdcd-4 Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin