Pdcd-1 Antibody (RMP1-30): sc-56200

Pdcd-1 Antibody (RMP1-30) is a rat monoclonal IgG2b antibody that detects the Pdcd-1 protein of mouse origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). Anti-Pdcd-1 antibody (RMP1-30) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Pdcd-1 protein, also known as Programmed Cell Death-1 or CD279, is a type I transmembrane receptor that plays a crucial role in regulating immune responses. Pdcd-1 is primarily located on the surface of activated T cells and B cells, where Pdcd-1 functions to inhibit excessive immune activation, thereby maintaining immune homeostasis. Expression on these immune cells is vital, as Pdcd-1 helps prevent autoimmunity and promotes tolerance to self-antigens. Notably, Pdcd-1 interacts with ligands such as PD-L1 and PD-L2, which are expressed on antigen-presenting cells and tumor cells, respectively. This interaction is significant in cancer immunotherapy, as blocking the Pdcd-1 pathway can enhance anti-tumor immunity. Expression of Pdcd-1 is induced upon T cell activation, and Pdcd-1 signaling involves the recruitment of tyrosine phosphatases, which modulate various immune responses. Using Pdcd-1 (RMP1-30) monoclonal antibody, researchers can gain insights into immune regulation mechanisms and potential therapeutic targets for enhancing immune responses in various diseases.

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Pdcd-1 Antibody (RMP1-30) References:

1. Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.  |  Nishimura, H., et al. 1999. Immunity. 11: 141-51. PMID: 10485649
2. New regulatory co-receptors: inducible co-stimulator and PD-1.  |  Okazaki, T., et al. 2002. Curr Opin Immunol. 14: 779-82. PMID: 12413529
3. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death.  |  Ishida, Y., et al. 1992. EMBO J. 11: 3887-95. PMID: 1396582
4. PD-1 inhibits T-cell receptor induced phosphorylation of the ZAP70/CD3zeta signalosome and downstream signaling to PKCtheta.  |  Sheppard, KA., et al. 2004. FEBS Lett. 574: 37-41. PMID: 15358536
5. The association between polymorphisms in the PDCD1 gene and the risk of cancer: A PRISMA-compliant meta-analysis.  |  Zhang, J., et al. 2016. Medicine (Baltimore). 95: e4423. PMID: 27749524
6. Epigenetic and Genetic Regulation of PDCD1 Gene in Cancer Immunology.  |  Mishra, A. and Verma, M. 2018. Methods Mol Biol. 1856: 247-254. PMID: 30178256
7. Inhibitory immune checkpoints PDCD-1 and LAG-3 hypermethylation may reduce the risk of colorectal cancer.  |  Zhang, Y., et al. 2021. Mol Med. 27: 114. PMID: 34544358
8. Lack of Association Between PDCD-1 Polymorphisms and Colorectal Cancer Risk: A Case-Control Study.  |  Lin, J., et al. 2022. Immunol Invest. 51: 1867-1882. PMID: 35499255
9. Evaluation of the possible association of PDCD-1 and LAG3 gene polymorphisms with hepatocellular carcinoma risk.  |  Wei, J., et al. 2023. BMC Med Genomics. 16: 92. PMID: 37131179
10. Expression of the PD-1 antigen on the surface of stimulated mouse T and B lymphocytes.  |  Agata, Y., et al. 1996. Int Immunol. 8: 765-72. PMID: 8671665
11. Role of the inositol phosphatase SHIP in negative regulation of the immune system by the receptor Fc(gamma)RIIB.  |  Ono, M., et al. 1996. Nature. 383: 263-6. PMID: 8805703
12. Immunoreceptor tyrosine-based inhibition motifs.  |  Vivier, E. and Daëron, M. 1997. Immunol Today. 18: 286-91. PMID: 9190115

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pdcd-1 Antibody (RMP1-30) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Pdcd-1 Antibody (RMP1-30) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pdcd-1 Antibody (RMP1-30) Specifications

Alternate Product Name: Pdcd-1

Product Clone Name: RMP1-30

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: Pdcd-1

Epitope Species: mouse

Product Reactivity/Detection: Pdcd-1

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, FCM

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Pdcd-1 Antibody (RMP1-30): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin