PDC-E2 Antibody (C-1): sc-166899

PDC-E2 Antibody (C-1) is a mouse monoclonal IgG3 antibody that detects PDC-E2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PDC-E2 plays a crucial role in cellular metabolism as a component of the pyruvate dehydrogenase multienzyme complex, which is essential for converting pyruvate into acetyl-CoA, a key substrate for the citric acid cycle. This metabolic pathway is vital for energy production and the synthesis of fatty acids, making PDC-E2 integral to maintaining cellular energy homeostasis. PDC-E2′s structure includes both an amino-terminal lipoyl-bearing domain and a carboxy-terminal catalytic domain, with the lipoyl domain being particularly important for enzymatic function. The presence of specific autoantibodies against PDC-E2 in patients with primary biliary cirrhosis highlights PDC-E2′s significance in autoimmune responses, as these autoantibodies can lead to the destruction of biliary epithelial cells, ultimately resulting in liver damage. Anti-PDC-E2 antibody (C-1) is therefore an invaluable tool for researchers studying the mechanisms of autoimmune diseases and metabolic disorders, providing insights into PDC-E2′s function and implications in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PDC-E2 Antibody (C-1) References:

1. Quantitative and functional analysis of PDC-E2-specific autoreactive cytotoxic T lymphocytes in primary biliary cirrhosis.  |  Kita, H., et al. 2002. J Clin Invest. 109: 1231-40. PMID: 11994412
2. Differing epitope selection of experimentally-induced and natural antibodies to a disease-specific autoantigen, the E2 subunit of pyruvate dehydrogenase complex (PDC-E2).  |  Rowley, MJ., et al. 1992. Int Immunol. 4: 1245-53. PMID: 1282029
3. Sidechain biology and the immunogenicity of PDC-E2, the major autoantigen of primary biliary cirrhosis.  |  Mao, TK., et al. 2004. Hepatology. 40: 1241-8. PMID: 15558739
4. Catalytic domain of PDC-E2 contains epitopes recognized by antimitochondrial antibodies in primary biliary cirrhosis.  |  Braun, S., et al. 2010. World J Gastroenterol. 16: 973-81. PMID: 20180236
5. Nuclear localization of pyruvate dehydrogenase complex-E2 (PDC-E2), a mitochondrial enzyme, and its role in signal transducer and activator of transcription 5 (STAT5)-dependent gene transcription.  |  Chueh, FY., et al. 2011. Cell Signal. 23: 1170-8. PMID: 21397011
6. Antibodies to post-translationally modified mitochondrial peptide PDC-E2(167-184) in type 1 diabetes.  |  Nuti, F., et al. 2018. Arch Biochem Biophys. 659: 66-74. PMID: 30266625
7. p-STAT3 is a PDC-E2 interacting partner in human cholangiocytes and hepatocytes with potential pathobiological implications.  |  Kilanczyk, E., et al. 2021. Sci Rep. 11: 21649. PMID: 34737337
8. Identification of a novel PDC-E2 epitope in primary biliary cholangitis: Application for engineered Treg therapy.  |  Tewari, R., et al. 2024. J Autoimmun. 149: 103327. PMID: 39476446
9. Abnormal expression of PDC-E2 on the apical surface of biliary epithelial cells in patients with antimitochondrial antibody-negative primary biliary cirrhosis.  |  Tsuneyama, K., et al. 1995. Hepatology. 22: 1440-6. PMID: 7590661
10. Random phage mimotopes recognized by monoclonal antibodies against the pyruvate dehydrogenase complex-E2 (PDC-E2).  |  Cha, S., et al. 1996. Proc Natl Acad Sci U S A. 93: 10949-54. PMID: 8855289

The preferred PDC-E2 antibody is PDC-E2 Antibody (B-2): sc-271534.