PCPE-2 CRISPR/Cas9 KO Plasmid (h): sc-411925
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PCPE-2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PCPE-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCPE-2 CRISPR/Cas9 KO Plasmid (h) | sc-411925 | 20 µg | $397.00 |
Overview
PCOLCE2 encodes procollagen C-endopeptidase enhancer protein 2 (PCPE-2), an extracellular matrix-associated glycoprotein that enhances BMP1/tolloid-like proteinase processing of procollagen C-propeptides, thereby promoting collagen fibrillogenesis and matrix maturation. By modulating collagen deposition and turnover, PCPE-2 contributes to tissue remodeling programs linked to fibroblast activation, wound repair, and regulation of stromal mechanics. Altered PCOLCE2 expression or PCPE-2 activity has been implicated in fibrotic remodeling and other pathologies characterized by dysregulated extracellular matrix organization. In human systems, PCPE-2 is therefore a useful node for interrogating collagen biosynthesis pathways and matrix-driven signaling crosstalk.
PCPE-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PCOLCE2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PCOLCE2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PCOLCE2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PCPE-2 protein expression.
This CRISPR knockout system enables efficient generation of PCOLCE2-deficient cell models for investigation of PCPE-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PCOLCE2 exon(s) critical for PCPE-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PCOLCE2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PCPE-2 CRISPR/Cas9 KO Plasmid (h) and PCPE-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PCOLCE2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PCPE-2 HDR Plasmid (h) and PCPE-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PCOLCE2 homology arms to support homology-directed repair at defined PCOLCE2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.