PCNA Antibody (PC10): sc-56. Fluorescent western blot analysis of PCNA Protein expression in HCT-116 (A), Raji (B), HeLa (C), MOLT-4 (D), NIH/3T3 (E) and KNRK (F) whole cell lysates. Blocked with UltraCruz® Blocking Reagent: sc-516214. Detection reagent used: m-IgG2a BP-CFL 647: sc-542738.
PCNA Antibody (PC10): sc-56
- PCNA (Clone PC10) is a mouse monoclonal IgG2a κ antibody, currently cited in 2,526 publications, and provided at 200 µg/ml
- The Calculated Molecular Weight of PCNA is ~29 kDa, however observed weight of PCNA is ~36kDa.
- Detects PCNA protein expressed at high levels in proliferating cells of mouse, rat, human, insect and S. pombe origin by WB, IP, IF, IHC(P) and FCM
- PCNA (proliferating cell nuclear antigen) is an auxiliary protein of DNA polymerase delta that assists in DNA replication and DNA repair.
- Antibodies for PCNA are antinuclear antibodies (ANAs) typically used to study cell proliferation, DNA replication, and cancer.
- Anti-PCNA Antibody (PC10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PCNA (PC10): sc-56.
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PCNA Antibody (PC10) is a mouse monoclonal IgG2a kappa light chain antibody. Proliferating Cell Nuclear Antigen (PCNA) is a critical nuclear protein involved in DNA replication and repair in eukaryotic cells. PCNA forms a ring structure that acts as a scaffold for recruiting proteins, supporting DNA polymerases during replication. PCNA plays key roles in DNA metabolism, chromatin remodeling, cell cycle regulation, and DNA repair pathways, ensuring high-fidelity DNA replication. PCNA is regulated by post-translational modifications, allowing dynamic response to DNA damage and maintenance of genomic integrity. Dysregulation of PCNA is linked to cancer, making PCNA an important biomarker for prognosis and a potential therapeutic target. PCNA monoclonal antibody (PC10) is used in various conjugated forms for IF and ELISA, providing clear nuclear staining during DNA replication. PCNA (PC10) antibody′s high specificity and sensitivity make this product useful for studying cell proliferation, DNA repair, and tumor growth, aiding in the development of therapeutic strategies for diseases related to abnormal cell proliferation.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PCNA Antibody (PC10) References:
- The contribution of E2F-regulated transcription to Drosophila PCNA gene function. | Thacker, SA., et al. 2003. Curr Biol. 13: 53-8. PMID: 12526745
- Germline mutations at microsatellite loci in homozygous and heterozygous mutants for mismatch repair and PCNA genes in Drosophila. | Baida, A., et al. 2003. DNA Repair (Amst). 2: 827-33. PMID: 12826283
- The human proliferating Cell nuclear antigen regulates transcriptional coactivator p300 activity and promotes transcriptional repression. | Hong, R. and Chakravarti, D. 2003. J Biol Chem. 278: 44505-13. PMID: 12937166
- Armadillo/Pangolin regulates PCNA and DREF promoter activities. | Kwon, E., et al. 2004. Biochim Biophys Acta. 1679: 256-62. PMID: 15358517
- GFP-PCNA as an S-phase marker in embryos during the first and subsequent cell cycles. | Kisielewska, J., et al. 2005. Biol Cell. 97: 221-9. PMID: 15584900
- Germline genomic instability in PCNA mutants of Drosophila: DNA fingerprinting and microsatellite analysis. | López, A., et al. 2005. Mutat Res. 570: 253-65. PMID: 15708584
- The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis. | Woods, AL., et al. 1991. Histopathology. 19: 21-7. PMID: 1680784
- Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form. | Waseem, NH. and Lane, DP. 1990. J Cell Sci. 96 (Pt 1): 121-9. PMID: 1695635
- The archaeal PCNA proteins. | Pan, M., et al. 2011. Biochem Soc Trans. 39: 20-4. PMID: 21265741
- Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites. | Bravo, R. and Macdonald-Bravo, H. 1987. J Cell Biol. 105: 1549-54. PMID: 2889739
- Identification of PCNA-interacting protein motifs in human DNA polymerase δ. | Khandagale, P., et al. 2020. Biosci Rep. 40: PMID: 32314787
- PCNA as Protein-Based Nanoruler for Sub-10 nm Fluorescence Imaging. | Helmerich, DA., et al. 2024. Adv Mater. 36: e2310104. PMID: 38009560
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCNA Antibody (PC10) | sc-56 | 200 µg/ml | $316.00 | |||
PCNA Antibody (PC10): m-IgG Fc BP-HRP Bundle | sc-528093 | 200 µg Ab; 10 µg BP | $354.00 | |||
PCNA Antibody (PC10): m-IgGκ BP-HRP Bundle | sc-520391 | 200 µg Ab, 40 µg BP | $354.00 | |||
PCNA Antibody (PC10): m-IgG2a BP-HRP Bundle | sc-547021 | 200 µg Ab; 10 µg BP | $354.00 | |||
PCNA Antibody (PC10) AC | sc-56 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PCNA Antibody (PC10) HRP | sc-56 HRP | 200 µg/ml | $316.00 | |||
PCNA Antibody (PC10) FITC | sc-56 FITC | 200 µg/ml | $330.00 | |||
PCNA Antibody (PC10) PE | sc-56 PE | 200 µg/ml | $343.00 | |||
PCNA Antibody (PC10) Alexa Fluor® 488 | sc-56 AF488 | 200 µg/ml | $357.00 | |||
PCNA Antibody (PC10) Alexa Fluor® 546 | sc-56 AF546 | 200 µg/ml | $357.00 | |||
PCNA Antibody (PC10) Alexa Fluor® 594 | sc-56 AF594 | 200 µg/ml | $357.00 | |||
PCNA Antibody (PC10) Alexa Fluor® 647 | sc-56 AF647 | 200 µg/ml | $357.00 | |||
PCNA Antibody (PC10) Alexa Fluor® 680 | sc-56 AF680 | 200 µg/ml | $357.00 | |||
PCNA Antibody (PC10) Alexa Fluor® 790 | sc-56 AF790 | 200 µg/ml | $357.00 | |||
PCNA Antibody (PC10) B | sc-56 B | 200 µg/ml | $320.00 | |||
PCNA Antibody (PC10) TRITC | sc-56 TRITC | 200 µg/ml | $320.00 | |||
PCNA Antibody (PC10) Alexa Fluor® 405 | sc-56 AF405 | 200 µg/ml | $357.00 |