PCIF1 Antibody (A-9): sc-374406
- PCIF1 Antibody (A-9) is a mouse monoclonal IgG1 κ PCIF1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 213-251 within an internal region of PCIF1 of human origin
- PCIF1 Antibody (A-9) is recommended for detection of PCIF1 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-PCIF1 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for PCIF1 Antibody (A-9) for WB and IHC(P) applications. This reagent is now offered in a bundle with PCIF1 Antibody (A-9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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PCIF1 Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects PCIF1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PCIF1 (A-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PCIF1, also known as Phosphorylated CTD-interacting factor 1 or C20orf67, is a 704 amino acid nuclear protein characterized by the presence of a WW domain. PCIF1 is expressed throughout various tissues and plays a crucial role in transcription elongation, particularly in coupling transcription to pre-mRNA processing by interacting with the phosphorylated carboxy terminal domain (CTD) of RNA polymerase II. Notably, PCIF1 also interacts with PDX-1 (pancreatic and duodenal homeobox-1), inhibiting PDX-1 activity and thereby regulating beta-cell differentiation, which is essential for normal pancreatic development. This interaction highlights PCIF1′s importance in pancreatic organogenesis and potential implications in diabetes pathogenesis, making PCIF1 a promising target for therapeutic interventions in diabetes management.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PCIF1 Antibody (A-9) References:
- The PDX-1 activation domain provides specific functions necessary for transcriptional stimulation in pancreatic beta-cells. | Peshavaria, M., et al. 2000. Mol Endocrinol. 14: 1907-17. PMID: 11117522
- Splicing and transcription-associated proteins PSF and p54nrb/nonO bind to the RNA polymerase II CTD. | Emili, A., et al. 2002. RNA. 8: 1102-11. PMID: 12358429
- PCIF1, a novel human WW domain-containing protein, interacts with the phosphorylated RNA polymerase II. | Fan, H., et al. 2003. Biochem Biophys Res Commun. 301: 378-85. PMID: 12565871
- Identification of PCIF1, a POZ domain protein that inhibits PDX-1 (MODY4) transcriptional activity. | Liu, A., et al. 2004. Mol Cell Biol. 24: 4372-83. PMID: 15121856
- Two conserved domains in PCIF1 mediate interaction with pancreatic transcription factor PDX-1. | Liu, A., et al. 2006. FEBS Lett. 580: 6701-6. PMID: 17126328
- Phosphorylation of the C-terminal domain of RNA polymerase II plays central roles in the integrated events of eucaryotic gene expression. | Hirose, Y. and Ohkuma, Y. 2007. J Biochem. 141: 601-8. PMID: 17405796
- Human phosphorylated CTD-interacting protein, PCIF1, negatively modulates gene expression by RNA polymerase II. | Hirose, Y., et al. 2008. Biochem Biophys Res Commun. 369: 449-55. PMID: 18294453
- RNA polymerases IIA and IIO have distinct roles during transcription from the TATA-less murine dihydrofolate reductase promoter. | Kang, ME. and Dahmus, ME. 1993. J Biol Chem. 268: 25033-40. PMID: 8227067
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCIF1 Antibody (A-9) | sc-374406 | 200 µg/ml | $322.00 | |||
PCIF1 Antibody (A-9): m-IgGκ BP-HRP Bundle | sc-522726 | 200 µg Ab, 40 µg BP | $361.00 | |||
PCIF1 Antibody (A-9) AC | sc-374406 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PCIF1 Antibody (A-9) HRP | sc-374406 HRP | 200 µg/ml | $322.00 | |||
PCIF1 Antibody (A-9) FITC | sc-374406 FITC | 200 µg/ml | $336.00 | |||
PCIF1 Antibody (A-9) PE | sc-374406 PE | 200 µg/ml | $349.00 | |||
PCIF1 Antibody (A-9) Alexa Fluor® 488 | sc-374406 AF488 | 200 µg/ml | $364.00 | |||
PCIF1 Antibody (A-9) Alexa Fluor® 546 | sc-374406 AF546 | 200 µg/ml | $364.00 | |||
PCIF1 Antibody (A-9) Alexa Fluor® 594 | sc-374406 AF594 | 200 µg/ml | $364.00 | |||
PCIF1 Antibody (A-9) Alexa Fluor® 647 | sc-374406 AF647 | 200 µg/ml | $364.00 | |||
PCIF1 Antibody (A-9) Alexa Fluor® 680 | sc-374406 AF680 | 200 µg/ml | $364.00 | |||
PCIF1 Antibody (A-9) Alexa Fluor® 790 | sc-374406 AF790 | 200 µg/ml | $364.00 | |||
PCIF1 (A-9) Neutralizing Peptide | sc-374406 P | 100 µg/0.5 ml | $69.00 |