PCGF1 Antibody (E-8): sc-515371
- PCGF1 Antibody (E-8) is a mouse monoclonal IgG1 κ PCGF1 antibody, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 128-247 mapping near the C-terminus of PCGF1 of human origin
- PCGF1 Antibody (E-8) is recommended for detection of PCGF1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PCGF1 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PCGF1 Antibody (E-8) for WB applications. These reagents are now offered in bundles with PCGF1 Antibody (E-8) (see ordering information below).
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PCGF1 Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects PCGF1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PCGF1 (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PCGF1, also known as polycomb group ring finger 1, plays a crucial role in regulating gene expression, particularly in maintaining the transcriptionally repressive state of developmental and cell proliferation genes. PCGF1 functions as a key component of the polycomb group multiprotein BCoR complex, which is essential for silencing genes such as Bcl-6 and the cyclin-dependent kinase inhibitor p21. PCGF1 includes a RING-type zinc finger motif, a conserved domain involved in the ubiquitination pathway, which serves as a critical mechanism for protein degradation and regulation within cells. PCGF1 promotes cell cycle progression and enhances cell proliferation, making this protein vital for normal cellular function and development. Ubiquitous expression and involvement in multiprotein complexes underscore PCGF1′s importance in various biological processes, making anti-PCGF1 antibody (E-8) an invaluable tool for researchers studying gene regulation and cell growth.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PCGF1 Antibody (E-8) References:
- NSPc1, a novel mammalian Polycomb gene, is expressed in neural crest-derived structures of the peripheral nervous system. | Nunes, M., et al. 2001. Mech Dev. 102: 219-22. PMID: 11287196
- NSPc1, a mainly nuclear localized protein of novel PcG family members, has a transcription repression activity related to its PKC phosphorylation site at S183. | Gong, Y., et al. 2005. FEBS Lett. 579: 115-21. PMID: 15620699
- Polycomb group and SCF ubiquitin ligases are found in a novel BCOR complex that is recruited to BCL6 targets. | Gearhart, MD., et al. 2006. Mol Cell Biol. 26: 6880-9. PMID: 16943429
- NSPc1 is a cell growth regulator that acts as a transcriptional repressor of p21Waf1/Cip1 via the RARE element. | Gong, Y., et al. 2006. Nucleic Acids Res. 34: 6158-69. PMID: 17088287
- Proteomics analysis of Ring1B/Rnf2 interactors identifies a novel complex with the Fbxl10/Jhdm1B histone demethylase and the Bcl6 interacting corepressor. | Sánchez, C., et al. 2007. Mol Cell Proteomics. 6: 820-34. PMID: 17296600
- Cooperation between EZH2, NSPc1-mediated histone H2A ubiquitination and Dnmt1 in HOX gene silencing. | Wu, X., et al. 2008. Nucleic Acids Res. 36: 3590-9. PMID: 18460542
- [Expression pattern of polycomb gene Nspc1 at the early developmental stage in zebrafish]. | Wu, XD., et al. 2008. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 30: 550-3. PMID: 19024384
- Side population in oral squamous cell carcinoma possesses tumor stem cell phenotypes. | Zhang, P., et al. 2009. Cancer Lett. 277: 227-34. PMID: 19185988
- mel-18, a Polycomb group-related mammalian gene, encodes a transcriptional negative regulator with tumor suppressive activity. | Kanno, M., et al. 1995. EMBO J. 14: 5672-8. PMID: 8521824
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCGF1 Antibody (E-8) | sc-515371 | 200 µg/ml | $316.00 | |||
PCGF1 Antibody (E-8): m-IgG Fc BP-HRP Bundle | sc-528031 | 200 µg Ab; 10 µg BP | $354.00 | |||
PCGF1 Antibody (E-8): m-IgG1 BP-HRP Bundle | sc-533404 | 200 µg Ab; 20 µg BP | $354.00 | |||
PCGF1 Antibody (E-8) AC | sc-515371 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PCGF1 Antibody (E-8) HRP | sc-515371 HRP | 200 µg/ml | $316.00 | |||
PCGF1 Antibody (E-8) FITC | sc-515371 FITC | 200 µg/ml | $330.00 | |||
PCGF1 Antibody (E-8) PE | sc-515371 PE | 200 µg/ml | $343.00 | |||
PCGF1 Antibody (E-8) Alexa Fluor® 488 | sc-515371 AF488 | 200 µg/ml | $357.00 | |||
PCGF1 Antibody (E-8) Alexa Fluor® 546 | sc-515371 AF546 | 200 µg/ml | $357.00 | |||
PCGF1 Antibody (E-8) Alexa Fluor® 594 | sc-515371 AF594 | 200 µg/ml | $357.00 | |||
PCGF1 Antibody (E-8) Alexa Fluor® 647 | sc-515371 AF647 | 200 µg/ml | $357.00 | |||
PCGF1 Antibody (E-8) Alexa Fluor® 680 | sc-515371 AF680 | 200 µg/ml | $357.00 | |||
PCGF1 Antibody (E-8) Alexa Fluor® 790 | sc-515371 AF790 | 200 µg/ml | $357.00 |