Pcf11 Antibody (C-9): sc-514158

Pcf11 Antibody (C-9) is a mouse monoclonal IgG3 kappa light chain antibody that detects Pcf11 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Pcf11 (C-9) antibody is available in a non-conjugated form, allowing for versatile experimental use. Pcf11, also known as cleavage and polyadenylation factor subunit homolog (S. cerevisiae), plays a vital role in the regulation of gene expression by facilitating the recruitment of polyadenylation machinery to elongating RNA polymerase II (RNAPII), which is essential for proper transcriptional termination. This 1,555 amino acid nuclear protein is a key component of the pre-mRNA cleavage complex II and promotes the dissociation of RNA polymerase II elongation complexes from DNA, thereby influencing the efficiency of transcription. Pcf11 contains a carboxy-terminal domain (CTD)-interaction domain that binds to the heptad repeats within the RNA polymerase II CTD in a phospho-dependent manner, highlighting its importance in the transcription cycle. The gene encoding Pcf11 is located on human chromosome 11, a region associated with various genetic disorders, including Jervell and Lange-Nielsen syndrome and Jacobsen syndrome, underscoring the significance of Pcf11 in both normal cellular function and disease.

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Pcf11 Antibody (C-9) References:

1. Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors.  |  de Vries, H., et al. 2000. EMBO J. 19: 5895-904. PMID: 11060040
2. Functional interaction of yeast pre-mRNA 3' end processing factors with RNA polymerase II.  |  Licatalosi, DD., et al. 2002. Mol Cell. 9: 1101-11. PMID: 12049745
3. Coupling of termination, 3' processing, and mRNA export.  |  Hammell, CM., et al. 2002. Mol Cell Biol. 22: 6441-57. PMID: 12192043
4. Recognition of RNA polymerase II carboxy-terminal domain by 3'-RNA-processing factors.  |  Meinhart, A. and Cramer, P. 2004. Nature. 430: 223-6. PMID: 15241417
5. Key features of the interaction between Pcf11 CID and RNA polymerase II CTD.  |  Noble, CG., et al. 2005. Nat Struct Mol Biol. 12: 144-51. PMID: 15665873
6. RNA polymerase II CTD phosphopeptides compete with RNA for the interaction with Pcf11.  |  Hollingworth, D., et al. 2006. RNA. 12: 555-60. PMID: 16497660
7. Structure of a nucleotide-bound Clp1-Pcf11 polyadenylation factor.  |  Noble, CG., et al. 2007. Nucleic Acids Res. 35: 87-99. PMID: 17151076
8. Transcription termination factor Pcf11 limits the processivity of Pol II on an HIV provirus to repress gene expression.  |  Zhang, Z., et al. 2007. Genes Dev. 21: 1609-14. PMID: 17606639
9. Human Pcf11 enhances degradation of RNA polymerase II-associated nascent RNA and transcriptional termination.  |  West, S. and Proudfoot, NJ. 2008. Nucleic Acids Res. 36: 905-14. PMID: 18086705

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pcf11 Antibody (C-9) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Pcf11 Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pcf11 Antibody (C-9) Specifications

Alternate Product Name: Pcf11

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 737-767

Epitope Target: Pcf11

Epitope Species: human

Product Reactivity/Detection: Pcf11

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Pcf11 Antibody (C-9): P: Blocking Peptide

The preferred Pcf11 antibody is Pcf11 Antibody (A-7): sc-515669.