PCDH17 CRISPR/Cas9 KO Plasmid (h): sc-405740
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PCDH17 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PCDH17 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCDH17 CRISPR/Cas9 KO Plasmid (h) | sc-405740 | 20 µg | $397.00 |
Overview
PCDH17 (protocadherin-17) is a calcium-dependent cell–cell adhesion molecule of the protocadherin family implicated in establishing and maintaining tissue architecture, particularly within the nervous system. By mediating homophilic interactions at the plasma membrane, PCDH17 contributes to neurite outgrowth, synaptic connectivity, and cell sorting processes that shape circuit formation. PCDH17-associated adhesion dynamics intersect with cytoskeletal remodeling and signaling pathways that regulate migration and differentiation, including Rho-family GTPase-linked processes. Dysregulated PCDH17 expression or epigenetic silencing has been reported in multiple tumor contexts and is studied for roles in altered adhesion, invasive behavior, and lineage-specific transcriptional programs.
PCDH17 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PCDH17 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PCDH17 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PCDH17 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PCDH17 protein expression.
This CRISPR knockout system enables efficient generation of PCDH17-deficient cell models for investigation of PCDH17 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PCDH17 exon(s) critical for PCDH17 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PCDH17 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PCDH17 CRISPR/Cas9 KO Plasmid (h) and PCDH17 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PCDH17 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PCDH17 HDR Plasmid (h) and PCDH17 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PCDH17 homology arms to support homology-directed repair at defined PCDH17 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.