PCDH15 Antibody (H-3): sc-377235
- PCDH15 Antibody (H-3) is a mouse monoclonal IgG3 κ PCDH15 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 781-1079 mapping within an internal region of PCDH15 of human origin
- PCDH15 Antibody (H-3) is recommended for detection of PCDH15 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PCDH15 Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for PCDH15 Antibody (H-3) for WB applications. This reagent is now offered in a bundle with PCDH15 Antibody (H-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
PCDH15 Antibody (H-3) is a mouse monoclonal IgG3 kappa light chain antibody that detects PCDH15 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PCDH15 antibody (H-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PCDH15, also known as protocadherin 15, is a critical calcium-dependent cell-adhesion protein that plays a vital role in maintaining normal cochlear and retinal function, making PCDH15 essential for auditory and visual processes. PCDH15 is primarily expressed in the testis, brain, lung, kidney, and spleen, and consists of 1,955 amino acids with a single-pass type I membrane structure featuring 11 cadherin domains. Multiple alternatively spliced isoforms of PCDH15 allow for functional diversity, which is crucial for establishing and maintaining neuronal connections in the brain. Defects in PCDH15 are linked to several conditions, including Usher syndrome type 1F, Usher syndrome type 1D/F, and non-syndromic sensorineural deafness autosomal recessive type 23, highlighting PCDH15′s significance in sensory function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PCDH15 Antibody (H-3) References:
- Mutations of the protocadherin gene PCDH15 cause Usher syndrome type 1F. | Ahmed, ZM., et al. 2001. Am J Hum Genet. 69: 25-34. PMID: 11398101
- Mutations in the novel protocadherin PCDH15 cause Usher syndrome type 1F. | Alagramam, KN., et al. 2001. Hum Mol Genet. 10: 1709-18. PMID: 11487575
- PCDH15 is expressed in the neurosensory epithelium of the eye and ear and mutant alleles are responsible for both USH1F and DFNB23. | Ahmed, ZM., et al. 2003. Hum Mol Genet. 12: 3215-23. PMID: 14570705
- Digenic inheritance of deafness caused by mutations in genes encoding cadherin 23 and protocadherin 15 in mice and humans. | Zheng, QY., et al. 2005. Hum Mol Genet. 14: 103-11. PMID: 15537665
- A new spontaneous mutation in the mouse protocadherin 15 gene. | Zheng, QY., et al. 2006. Hear Res. 219: 110-20. PMID: 16887306
- Large genomic rearrangements within the PCDH15 gene are a significant cause of USH1F syndrome. | Le Guédard, S., et al. 2007. Mol Vis. 13: 102-7. PMID: 17277737
- Promoter, alternative splice forms, and genomic structure of protocadherin 15. | Alagramam, KN., et al. 2007. Genomics. 90: 482-92. PMID: 17706913
- Usher syndromes due to MYO7A, PCDH15, USH2A or GPR98 mutations share retinal disease mechanism. | Jacobson, SG., et al. 2008. Hum Mol Genet. 17: 2405-15. PMID: 18463160
- Gene structure and mutant alleles of PCDH15: nonsyndromic deafness DFNB23 and type 1 Usher syndrome. | Ahmed, ZM., et al. 2008. Hum Genet. 124: 215-23. PMID: 18719945
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCDH15 Antibody (H-3) | sc-377235 | 200 µg/ml | $316.00 | |||
PCDH15 Antibody (H-3): m-IgGκ BP-HRP Bundle | sc-523244 | 200 µg Ab, 40 µg BP | $354.00 | |||
PCDH15 Antibody (H-3) AC | sc-377235 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PCDH15 Antibody (H-3) HRP | sc-377235 HRP | 200 µg/ml | $316.00 | |||
PCDH15 Antibody (H-3) FITC | sc-377235 FITC | 200 µg/ml | $330.00 | |||
PCDH15 Antibody (H-3) PE | sc-377235 PE | 200 µg/ml | $343.00 | |||
PCDH15 Antibody (H-3) Alexa Fluor® 488 | sc-377235 AF488 | 200 µg/ml | $357.00 | |||
PCDH15 Antibody (H-3) Alexa Fluor® 546 | sc-377235 AF546 | 200 µg/ml | $357.00 | |||
PCDH15 Antibody (H-3) Alexa Fluor® 594 | sc-377235 AF594 | 200 µg/ml | $357.00 | |||
PCDH15 Antibody (H-3) Alexa Fluor® 647 | sc-377235 AF647 | 200 µg/ml | $357.00 | |||
PCDH15 Antibody (H-3) Alexa Fluor® 680 | sc-377235 AF680 | 200 µg/ml | $357.00 | |||
PCDH15 Antibody (H-3) Alexa Fluor® 790 | sc-377235 AF790 | 200 µg/ml | $357.00 |