PCB Antibody (F-3): sc-365491
- PCB Antibody (F-3) is a mouse monoclonal IgG3 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1015-1041 near the C-terminus of PCB of human origin
- recommended for detection of PCB of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and bovine
- m-IgG3 BP-HRP is the preferred secondary detection reagent for PCB Antibody (F-3) for WB applications. This reagent is now offered in a bundle with PCB Antibody (F-3) (see ordering information below).
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PCB Antibody (F-3) is a mouse monoclonal IgG3 antibody that detects pyruvate carboxylase (PCB) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PCB antibody (F-3) recognizes pyruvate carboxylase, a crucial mitochondrial enzyme responsible for catalyzing the conversion of pyruvate to oxaloacetate, a key step in gluconeogenesis and the citric acid cycle. This enzymatic function is vital for maintaining glucose homeostasis and energy production, particularly in tissues with high metabolic demands, such as the liver and brain. The proper functioning of pyruvate carboxylase is essential, as deficiencies can lead to severe metabolic disorders, including lactic acidosis and hypoglycemia, which can have profound effects on neurological development and function. The PCB gene, located on the long arm of chromosome 11, is subject to mutations that can disrupt activity, highlighting the importance of pyruvate carboxylase in metabolic regulation and overall cellular health. With an ability to bind biotin and interact with ATP and pyruvate, pyruvate carboxylase plays a pivotal role in cellular metabolism, making PCB (F-3) antibody an invaluable tool for researchers studying metabolic pathways and related disorders.
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PCB Antibody (F-3) References:
- Does P-glycoprotein play a role in clinical resistance of malignant astrocytoma? | Ashmore, SM., et al. 1999. Anticancer Drugs. 10: 861-72. PMID: 10630353
- Pyruvate carboxylase in genetic obesity. | Lynch, CJ., et al. 1992. Am J Physiol. 262: E608-18. PMID: 1375435
- Inhibitors of Pyruvate Carboxylase. | Zeczycki, TN., et al. 2010. Open Enzym Inhib J. 3: 8-26. PMID: 22180764
- Impact of hematopoietic cyclooxygenase-1 deficiency on obesity-linked adipose tissue inflammation and metabolic disorders in mice. | Saraswathi, V., et al. 2013. Metabolism. 62: 1673-85. PMID: 23987235
- Erianin suppresses proliferation and migration of cancer cells in a pyruvate carboxylase-dependent manner. | Hong, J., et al. 2022. Fitoterapia. 157: 105136. PMID: 35093481
- Hypoxia-mediated repression of pyruvate carboxylase drives immunosuppression. | Coleman, MF., et al. 2024. Breast Cancer Res. 26: 96. PMID: 38849928
- Enzymatic and immunological studies on pyruvate carboxylase in livers and liver tumors. | Chang, LO. and Morris, HP. 1973. Cancer Res. 33: 2034-41. PMID: 4353477
- A homogeneous enzyme immunoassay with avidin-ligand conjugate as the enzyme-modulator. | Bacquet, C. and Twumasi, DY. 1984. Anal Biochem. 136: 487-90. PMID: 6372543
- MDR1 gene-specific monoclonal antibody C494 cross-reacts with pyruvate carboxylase. | Rao, VV., et al. 1994. Cancer Res. 54: 1536-41. PMID: 7511043
- Disorders of pyruvate carboxylase and the pyruvate dehydrogenase complex. | Robinson, BH., et al. 1996. J Inherit Metab Dis. 19: 452-62. PMID: 8884569
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCB Antibody (F-3) | sc-365491 | 200 µg/ml | $322.00 | |||
PCB Antibody (F-3): m-IgG3 BP-HRP Bundle | sc-550430 | 200 µg Ab; 40 µg BP | $361.00 | |||
PCB (F-3) Neutralizing Peptide | sc-365491 P | 100 µg/0.5 ml | $69.00 |