Pbx 1/2/3/4 Antibody (F-3): sc-28313

Pbx 1/2/3/4 Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Pbx 1/2/3/4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Pbx 1/2/3/4 (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pbx proteins are integral members of the TALE (three amino acid loop extension) family of homeodomain-containing proteins, which play crucial roles in regulating gene expression during development and differentiation. Notably, Pbx 1, 2, 3, and 4 are involved in forming protein complexes that interact with other transcription factors, such as E2A proteins, which are essential for hematopoiesis and lymphocyte development. The interaction between Pbx proteins and these transcription factors is vital for proper regulation of genes involved in cell fate determination and differentiation, highlighting Pbx proteins′ importance in both normal development and disease pathogenesis, including acute leukemias. Pbx 1, 2, and 3 exhibit high sequence homology, particularly in homeobox domains, while Pbx 4 is uniquely expressed in testis, specifically in spermatocytes during meiosis. The ubiquitous expression of Pbx genes in various tissues underscores their fundamental role in cellular processes, making anti-Pbx 1/2/3/4 antibody (F-3) an essential tool for researchers studying gene regulation and developmental biology.

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Pbx 1/2/3/4 Antibody (F-3) References:

1. Expression of Meis and Pbx genes and their protein products in the developing telencephalon: implications for regional differentiation.  |  Toresson, H., et al. 2000. Mech Dev. 94: 183-7. PMID: 10842069
2. Pbx4, a new Pbx family member on mouse chromosome 8, is expressed during spermatogenesis.  |  Wagner, K., et al. 2001. Mech Dev. 103: 127-31. PMID: 11335119
3. Retinoic acid regulates the expression of PBX1, PBX2, and PBX3 in P19 cells both transcriptionally and post-translationally.  |  Qin, P., et al. 2004. J Cell Biochem. 92: 147-63. PMID: 15095411
4. Pbx1/Pbx2 requirement for distal limb patterning is mediated by the hierarchical control of Hox gene spatial distribution and Shh expression.  |  Capellini, TD., et al. 2006. Development. 133: 2263-73. PMID: 16672333
5. PBX2 and PBX3, new homeobox genes with extensive homology to the human proto-oncogene PBX1.  |  Monica, K., et al. 1991. Mol Cell Biol. 11: 6149-57. PMID: 1682799
6. Chromosomal translocation t(1;19) results in synthesis of a homeobox fusion mRNA that codes for a potential chimeric transcription factor.  |  Nourse, J., et al. 1990. Cell. 60: 535-45. PMID: 1967982
7. A new homeobox gene contributes the DNA binding domain of the t(1;19) translocation protein in pre-B ALL.  |  Kamps, MP., et al. 1990. Cell. 60: 547-55. PMID: 1967983
8. Transcription factor PBX4 regulates limb development and haematopoiesis in mice.  |  Ning, Y., et al. 2024. Cell Prolif. 57: e13580. PMID: 38230761
9. PBX1 and PBX3 transcription factors regulate SHH expression in the Frontonasal Ectodermal Zone through complementary mechanisms.  |  Mok, CH., et al. 2024. bioRxiv.. PMID: 38895322
10. Fusion with E2A converts the Pbx1 homeodomain protein into a constitutive transcriptional activator in human leukemias carrying the t(1;19) translocation.  |  Lu, Q., et al. 1994. Mol Cell Biol. 14: 3938-48. PMID: 7910944
11. Transformation properties of the E2a-Pbx1 chimeric oncoprotein: fusion with E2a is essential, but the Pbx1 homeodomain is dispensable.  |  Monica, K., et al. 1994. Mol Cell Biol. 14: 8304-14. PMID: 7969166
12. Fusion with E2A alters the transcriptional properties of the homeodomain protein PBX1 in t(1;19) leukemias.  |  LeBrun, DP. and Cleary, ML. 1994. Oncogene. 9: 1641-7. PMID: 8183558

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pbx 1/2/3/4 Antibody (F-3) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-Pbx 1/2/3/4 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pbx 1/2/3/4 Antibody (F-3) Specifications

Alternate Product Name: pan-PBX

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-260

Epitope Target: Pbx 1

Epitope Species: human

Product Reactivity/Detection: Pbx 1, Pbx 2, Pbx 3 and Pbx 4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Pbx 1/2/3/4 Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent