Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PBR Double Nickase Plasmid (h): sc-401624-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PBR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PBR Double Nickase Plasmid (h) and PBR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TSPO. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PBR Antibody (3D8-B2): sc-293216
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PBR Double Nickase Plasmid (h) | sc-401624-NIC | 20 µg | $410.00 | |||
PBR Double Nickase Plasmid (h2) | sc-401624-NIC-2 | 20 µg | $410.00 |
TSPO, also known as peripheral benzodiazepine receptor (PBR), is an 18 kDa mitochondrial outer membrane protein that participates in cholesterol transport, steroidogenesis, and regulation of mitochondrial permeability and bioenergetics. It is functionally linked to mitochondrial stress responses, reactive oxygen species homeostasis, and apoptotic signaling, and is widely used as a molecular marker of microglial activation and neuroinflammation. Altered TSPO expression and mitochondrial localization have been associated with inflammatory and neurodegenerative processes, as well as cancer cell metabolism and proliferation. As a mitochondria-associated hub, TSPO provides a tractable entry point for studying crosstalk between lipid trafficking, oxidative stress, and innate immune signaling.
PBR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TSPO locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TSPO. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TSPO function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TSPO-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.