PBR CRISPR/Cas9 KO Plasmid (m): sc-419383
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PBR CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PBR genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PBR CRISPR/Cas9 KO Plasmid (m) | sc-419383 | 20 µg | $397.00 |
Overview
Mouse Tspo encodes the peripheral benzodiazepine receptor (PBR), an 18 kDa protein primarily localized to the outer mitochondrial membrane that binds cholesterol and porphyrins and supports mitochondrial homeostasis. PBR participates in cholesterol transport and steroidogenic metabolism, regulates mitochondrial permeability and apoptosis-associated signaling, and influences reactive oxygen species balance and inflammatory responses. Tspo/PBR expression is enriched in activated microglia and peripheral immune cells and is widely used as a marker of neuroinflammation, with additional relevance to mitochondrial dysfunction observed in neurodegenerative disorders and metabolic stress contexts. These functions make Tspo a useful node for dissecting mitochondria-linked signaling networks that couple cellular stress to immune activation and tissue remodeling.
PBR CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tspo gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tspo together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tspo open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PBR protein expression.
This CRISPR knockout system enables efficient generation of Tspo-deficient cell models for investigation of PBR signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tspo exon(s) critical for PBR function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tspo genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PBR CRISPR/Cas9 KO Plasmid (m) and PBR CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tspo locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PBR HDR Plasmid (m) and PBR HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tspo homology arms to support homology-directed repair at defined Tspo target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.