paxillin Antibody (177): sc-136297

paxillin Antibody (177) is a mouse monoclonal IgG1 antibody raised against amino acids 1-557, representing the full-length paxillin protein of chicken origin. Paxillin, also known as PXN, is a focal adhesion phosphoprotein localized to the cytoskeleton, playing a crucial role in signal transduction pathways that regulate cell morphology, motility, and survival. Paxillin functions as a scaffold protein, recruiting structural and signaling molecules to focal adhesions, which are essential contact points between cells and the extracellular matrix. Phosphorylation of paxillin occurs in response to stimuli such as platelet-derived growth factor (PDGF) treatment and v-src transformation; kinases like focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2) can phosphorylate paxillin on specific tyrosine residues, notably Tyr118. This phosphorylation modulates paxillin′s interactions and is significant for various cellular processes, although FAK phosphorylation is not required for paxillin′s recruitment to adhesion sites. During mitosis, paxillin levels are reduced through proteolytic downregulation, and paxillin is alternatively phosphorylated on serine residues instead of tyrosine and serine, indicating a role in cell cycle regulation. anti-paxillin antibody (177) is reactive with paxillin from mouse, rat, human, avian, bovine, and canine origins in applications like western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF), making anti-paxillin antibody (177) a valuable tool for studying paxillin′s function across different species. Understanding paxillin′s role is important because alterations in paxillin expression or function are associated with diseases such as cancer, where paxillin can influence tumor cell migration and metastasis. Thus, paxillin monoclonal antibody (177) serves as a critical resource for researchers investigating cell adhesion, migration, and signal transduction pathways related to paxillin.

The preferred paxillin antibody is paxillin Antibody (B-2): sc-365379.