Pax-8 CRISPR/Cas9 KO Plasmid (h): sc-401757

Overview

PAX8 encodes Pax-8, a paired box transcription factor that regulates lineage specification and organogenesis, with prominent roles in thyroid follicular development and epithelial differentiation in the kidney and Müllerian system. Pax-8 binds sequence-specific DNA motifs to coordinate transcriptional programs controlling cell identity, proliferation, and survival, and it cooperates with other transcriptional regulators to shape chromatin and gene expression networks. In adult tissues, PAX8 helps maintain differentiated states, and dysregulated expression or rearrangements have been associated with altered transcriptional control in several epithelial malignancies, making it a frequent marker in tumor origin studies. These functions position PAX8 as a useful node for investigating developmental gene regulatory circuits, epithelial plasticity, and lineage-tracing signatures in human cell models.

Pax-8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PAX8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PAX8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PAX8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Pax-8 protein expression.

This CRISPR knockout system enables efficient generation of PAX8-deficient cell models for investigation of Pax-8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting PAX8 exon(s) critical for Pax-8 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple PAX8 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Pax-8 CRISPR/Cas9 KO Plasmid (h) and Pax-8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PAX8 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Pax-8 HDR Plasmid (h) and Pax-8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PAX8 homology arms to support homology-directed repair at defined PAX8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.