Pax-3 Antibody (F-2): sc-376204

Pax-3 Antibody (F-2) is a mouse monoclonal IgG3 antibody that detects Pax-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Pax-3 antibody (F-2) is available as the non-conjugated form, which is crucial for studying Pax-3′s role in developmental biology. Pax-3 protein is a vital transcription factor that plays a significant role in early neurogenesis, particularly in the development of the central nervous system. Pax-3 contains both a paired domain and a paired-type homeodomain, which are essential for DNA-binding capabilities, allowing regulation of target genes involved in cell differentiation and proliferation. Pax-3 expression is primarily localized to mitotic cells in the ventricular zone of the developing spinal cord, as well as in specific regions of the hindbrain, midbrain, and diencephalon. During embryonic development, Pax-3 is also expressed in neural crest cells, which are critical for the formation of various structures, including the craniofacial mesectoderm and limb mesenchyme. Mutations in Pax-3 have been linked to Waardenburg syndrome, a genetic condition characterized by hearing loss and pigmentation abnormalities, highlighting Pax-3′s importance in both normal development and disease.

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Pax-3 Antibody (F-2) References:

1. Waardenburg's syndrome patients have mutations in the human homologue of the Pax-3 paired box gene.  |  Tassabehji, M., et al. 1992. Nature. 355: 635-6. PMID: 1347148
2. Gene expression signatures identify rhabdomyosarcoma subtypes and detect a novel t(2;2)(q35;p23) translocation fusing PAX3 to NCOA1.  |  Wachtel, M., et al. 2004. Cancer Res. 64: 5539-45. PMID: 15313887
3. Pax-3, a novel murine DNA binding protein expressed during early neurogenesis.  |  Goulding, MD., et al. 1991. EMBO J. 10: 1135-47. PMID: 2022185
4. Autophagy-dependent crosstalk between GILT and PAX-3 influences radiation sensitivity of human melanoma cells.  |  Hathaway-Schrader, JD., et al. 2018. J Cell Biochem. 119: 2212-2221. PMID: 28857256
5. The regenerative potential of Pax3/Pax7 on skeletal muscle injury.  |  Azhar, M., et al. 2022. J Genet Eng Biotechnol. 20: 143. PMID: 36251225
6. Loss of Pax3 causes reduction of melanocytes in the developing mouse cochlea.  |  Udagawa, T., et al. 2024. Sci Rep. 14: 2210. PMID: 38278860
7. Isolation of two isoforms of the PAX3 gene transcripts and their tissue-specific alternative expression in human adult tissues.  |  Tsukamoto, K., et al. 1994. Hum Genet. 93: 270-4. PMID: 7545913
8. Regulation of Pax-3 expression in the dermomyotome and its role in muscle development.  |  Goulding, M., et al. 1994. Development. 120: 957-71. PMID: 7600971
9. Chromosomal localization of seven PAX genes and cloning of a novel family member, PAX-9.  |  Stapleton, P., et al. 1993. Nat Genet. 3: 292-8. PMID: 7981748
10. Mutations in the paired domain of the human PAX3 gene cause Klein-Waardenburg syndrome (WS-III) as well as Waardenburg syndrome type I (WS-I).  |  Hoth, CF., et al. 1993. Am J Hum Genet. 52: 455-62. PMID: 8447316
11. Epistatic relationship between Waardenburg syndrome genes MITF and PAX3.  |  Watanabe, A., et al. 1998. Nat Genet. 18: 283-6. PMID: 9500554
12. The role of Pax3 and Pax7 in development and cancer.  |  Mansouri, A. 1998. Crit Rev Oncog. 9: 141-9. PMID: 9973247

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pax-3 Antibody (F-2) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Pax-3 Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pax-3 Antibody (F-2) Specifications

Alternate Product Name: PAX3

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃

Amino Acids: 387-425

Epitope Target: Pax-3

Epitope Species: human

Product Reactivity/Detection: Pax-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Pax-3 Antibody (F-2): P: Blocking Peptide, X: TransCruz Reagent

The preferred Pax-3/7 antibody is Pax-3/7 Antibody (B-5): sc-365843.